https://scholars.lib.ntu.edu.tw/handle/123456789/556995
標題: | Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells | 作者: | Chen J.-F. Tsai Y.-T. Lai Y.-H. Lin C.-C. Chou H.-C. WEN-HUNG KUO MEI-LAN KO Wei Y.-S. Wang Y.-S. Lin M.-W. Chen Y.-J. Lee Y.-R. Chan H.-L. |
關鍵字: | 2D-DIGE; Antrodia Cinnamomea; Liver cancer; MALDI-TOF MS; Proteomics | 公開日期: | 2020 | 出版社: | Elsevier B.V. | 卷: | 187 | 來源出版物: | Journal of Pharmaceutical and Biomedical Analysis | 摘要: | Antrodia Cinnamomea is a fungus species widely used as a herb medicine for hypertension, cancer and handover. Nevertheless, the biological roles of Antrodia Cinnamomea on the molecular mechanism of liver cancer are not entirely understood. To determine whether Antrodia Cinnamomea is able to be used for the treatment of liver cancer and its molecular mechanism, we examined the effect of Antrodia Cinnamomea on the differential proteomic patterns in liver cancer cell lines HepG2 and C3A as well as in Chang's liver cell, a normal liver cell, using quantitative proteomic approach. The proteomic analysis demonstrated that abundance of 82, 125 and 125 proteins was significantly altered in Chang's liver cells, C3A and HepG2, respectively. The experimental outcomes also demonstrated that Antrodia Cinnamomea-induced cytotoxicity in liver cancer cells mostly involved dysregulation of protein folding, cytoskeleton regulation, redox-regulation, glycolysis pathway as well as transcription regulation. Further analysis also revealed that Antrodia Cinnamomea promoted misfolding of intracellular proteins and dysregulate of cellular redox-balance resulting in ER-stress. To sum up our studies demonstrated that the proteomic strategy used in this study offered a tool to investigate the molecular mechanisms of Antrodia Cinnamomea-induced liver cancer cytotoxicity. The proteomic results might be further evaluated as prospective targets in liver cancer treatment. ? 2020 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085031910&doi=10.1016%2fj.jpba.2020.113142&partnerID=40&md5=3a311f96465408d160f5bb2c24430690 https://scholars.lib.ntu.edu.tw/handle/123456789/556995 |
ISBN: | 32460214 | ISSN: | 0731-7085 | DOI: | 10.1016/j.jpba.2020.113142 | SDG/關鍵字: | acetyl coenzyme A acetyltransferase; activating transcription factor 6; alcohol; aldehyde reductase; antineoplastic agent; Antrodia camphorata extract; argonaute 2 protein; beta enolase; calnexin; cell protein; chaperonin containing TCP1; ERO1 like protein alpha; fungal extract; galectin 1; glucose 6 phosphate 1 dehydrogenase; glucose 6 phosphate dehydrogenase; glucose regulated protein; glyceraldehyde 3 phosphate dehydrogenase; growth arrest and DNA damage inducible protein 153; isocitrate dehydrogenase; nuclear factor kappa B repressing factor; nucleophosmin; peroxiredoxin 1; protein IRE1; Ran protein; superoxide dismutase; T complex protein 1 subunit epsilon; transaldolase; unclassified drug; vimentin; X box binding protein 1; antineoplastic agent; Antrodia camphorata; Article; cancer cell; cell death; cell growth; cell viability; Chang Liver cell line; concentration response; controlled study; cytoskeleton; drug cytotoxicity; drug effect; endoplasmic reticulum stress; glycolysis; Hep-G2 cell line; Hep-G2/C3A cell line; herbal medicine; human; IC50; immunoblotting; liver cancer; liver cancer cell line; matrix assisted laser desorption ionization time of flight mass spectrometry; molecular biology; molecular pathology; MTT assay; oxidation reduction reaction; priority journal; protein expression; protein folding; protein misfolding; protein protein interaction; proteomics; quantitative analysis; regulatory mechanism; signal transduction; transcription regulation; two dimensional difference gel electrophoresis; unfolded protein response; cell line; chemistry; endoplasmic reticulum stress; liver cell; liver tumor; metabolism; Polyporales; tumor cell line; Antineoplastic Agents; Cell Line; Cell Line, Tumor; Cytoskeleton; Endoplasmic Reticulum Stress; Hep G2 Cells; Hepatocytes; Humans; Liver Neoplasms; Oxidation-Reduction; Polyporales; Protein Folding; Proteomics |
顯示於: | 醫學系 |
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