https://scholars.lib.ntu.edu.tw/handle/123456789/557520
標題: | Osteogenic differentiation of mesenchymal stem cells derived from bone marrow of patients with myeloproliferative disorders | 作者: | Lee, Hsuan-Shu JIH-LUH TANG Chiou L.-L. KEH-SUNG TSAI Jiang C.-C. PAN-CHYR YANG PEI-MING YANG GUAN-TARN HUANG |
公開日期: | 2002 | 卷: | 101 | 期: | 2 | 起(迄)頁: | 124-128 | 來源出版物: | Journal of the Formosan Medical Association | 摘要: | Background: It has been frequently reported that culture-expanded mesenchymal stem cells from bone marrow of healthy donors can be induced to differentiate to osteocytic lineage. This study examined the potential for osteogenic differentiation of mesenchymal stem cells obtained from two patients with myeloproliferative disorders. Methods: Mesenchymal stem cells were derived from bone marrow aspirates obtained in an outpatient clinic from two patients, one with polycythemia vera and the other with essential thrombocythemia. Nucleated bone marrow cells were directly cultured in flasks. Adherent fibroblastic cells in monolayers were isolated by removing nonadherent cells during medium changes. Osteogenic differentiation was induced in expanded adherent cells for 2 weeks in osteogenic medium containing 100 nmol/L dexamethasone, 10 mmol/L β-glycerophosphate, and 0.05 mmol/L L-ascorbic acid-2-phosphate. Osteogenic differentiation was evaluated by alkaline phosphatase staining and determination of calcium in deposited minerals on culture plates. The expression of osteopontin mRNA was determined by reverse transcription-polymerase chain reaction. Results: After induction in osteogenic medium, the expression of alkaline phosphatase in mesenchymal stem cells become more intense. Induced alkaline phosphatase-positive cells assumed an irregular shape with multiple spiculate projections, while uninduced alkaline phosphatase-positive cells had a flattened polygonal shape or were elongated. Calcium deposition on the plates of induced cells was 0.39 ± 0.03 μmol/well in cells from the patient with polycythemia vera and 0.54 ± 0.03 μmol/well in cells from the patients with essential thrombocythemia, but was not detectable in uninduced cells from either patient. Induction by osteogenic medium markedly increased the expression of osteopontin mRNA in stem cells derived from both patients. Conclusions: In this study, mesenchymal stem cells obtained from aspiration of bone marrow in patients with myeloproliferative disorders were expanded by culture. After osteogenic induction, these cells were shown to be able to differentiate into osteocytic lineage in vitro. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036305534&partnerID=40&md5=0a5533f4b5d8845a78f6e20ff4d6d618 https://scholars.lib.ntu.edu.tw/handle/123456789/557520 |
ISSN: | 0929-6646 | SDG/關鍵字: | alkaline phosphatase; ascorbic acid 2 phosphate; calcium; dexamethasone; glycerophosphate; messenger RNA; mineral; osteopontin; adult; article; bone marrow biopsy; case report; cell adhesion; cell culture; cell differentiation; cell isolation; cell lineage; cell shape; cell structure; concentration (parameters); controlled study; culture medium; enzyme histochemistry; female; fibroblast; hematopoietic stem cell; human; human cell; male; mesenchyme cell; monolayer culture; myeloproliferative disorder; osteoblast; osteocyte; outpatient department; polycythemia vera; protein expression; reverse transcription polymerase chain reaction; stem cell transplantation; thrombocythemia; Cell Differentiation; Fibroblasts; Humans; Mesoderm; Polycythemia Vera; Thrombocythemia, Hemorrhagic; Tumor Cells, Cultured |
顯示於: | 醫學系 |
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