https://scholars.lib.ntu.edu.tw/handle/123456789/558325
標題: | Fibroblast Growth Factor Receptor Inhibitors Reduce Adipogenesis of Orbital Fibroblasts and Enhance Myofibroblastic Differentiation in Graves' Orbitopathy | 作者: | SHYANG-RONG SHIH SHU-LANG LIAO Shih, Chih-Wei YI-HSUAN WEI Lu, Ting-Xuan Chou, Chien-Hsiang Yen, Er-Yen YI-CHENG CHANG CHIA-CHI LIN Chi, Yu-Chiao WEI-SHIUNG YANG FENG-CHIAO TSAI |
關鍵字: | Adipogenesis; Graves’ orbitopathy; fibroblast growth factor receptors; fibroblast growth factors; myofibroblast | 公開日期: | 2-一月-2021 | 卷: | 29 | 期: | 1 | 來源出版物: | Ocular immunology and inflammation | 摘要: | Purpose: Orbital fibroblasts are involved in pathogenesis of Graves' orbitopathy (GO). Fibroblast growth factor (FGF) affects fibroblasts of GO. This study aims to investigate the roles of FGF and FGF receptor (FGFR) in GO.Methods: Serum FGF proteins and orbital fibroblast FGFR proteins and mRNAs were measured in GO patients and controls. Orbital fibroblasts of GO were cultured and accessed for changes in proliferation (by nuclei number and MTT), myofibroblastic differentiation (by α-SMA), and adipogenesis (by oil droplets using Oil Red O stain) under FGF1 with or without FGFR inhibitors (FGFRi).Results: Serum FGF1 and FGF2 were increased in GO patients. FGFR1 was the most abundantly expressed FGFR in GO orbital fibroblasts. FGF1 increased GO fibroblast proliferation/adipogenesis and suppressed myofibroblastic differentiation, while FGFRi reversed these effects.Conclusion: FGF signaling may be involved in GO pathogenesis. Manipulation of FGF-FGFR pathway for GO treatment is worthy of further investigation.Registration number on Clinicaltrials.gov: NCT03324022. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/558325 | ISSN: | 09273948 | DOI: | 10.1080/09273948.2019.1672196 | SDG/關鍵字: | cyclosporine; fibroblast growth factor 1; fibroblast growth factor 2; fibroblast growth factor receptor 1; fibroblast growth factor receptor 2; fibroblast growth factor receptor 3; fibroblast growth factor receptor 4; immunoglobulin; messenger RNA; methyl |
顯示於: | 醫學系 |
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