https://scholars.lib.ntu.edu.tw/handle/123456789/559124
標題: | The Microfluidic Microwell Device Integrating Surface Enhanced Raman Scattering for Bacteria Enrichment and in Situ Antibiotic Susceptibility Test | 作者: | Liao, C.-C. Huang, H.-K. Chen, Y.-Z. NIEN-TSU HUANG |
關鍵字: | Antibiotic susceptibility test; Microfluidics; Microwell; Surface-enhance Raman scattering | 公開日期: | 2020 | 卷: | 2020-January | 起(迄)頁: | 1048-1051 | 來源出版物: | Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) | 摘要: | To ensure appropriate antibiotics treatment, antibiotic susceptibility test (AST) is a common method in clinical therapies for selecting proper antibiotic treatment and preventing misuse or overuse of antibiotics. However, the existing method still undergoes several obstacles, such as time-consuming, label-intensive process and lack of accuracy. Here, we develop a microfluidics platform integrating microwell with surface-enhanced Raman scattering (SERS) technology for low concentration bacteria encapsulation followed by in situ AST. The microwell can greatly increase the effective bacteria concentration and SERS technology can enable a rapidly and highly specific molecular detection from bacteria-secreted metabolites. Combing above features, the microwell-SERS platform can achieve label-free, low concentration (103 CFU/mL) bacteria detection and has the potential to provide AST reference in clinical diagnosis. © 2020 IEEE. |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85083279843&partnerID=40&md5=7b732db2e9c19866d8f07625c7494329 https://scholars.lib.ntu.edu.tw/handle/123456789/559124 |
ISSN: | 10846999 | DOI: | 10.1109/MEMS46641.2020.9056435 | SDG/關鍵字: | Antibiotics; Bacteria; Diagnosis; Mechanics; MEMS; Metabolites; Microchannels; Microfluidics; Raman scattering; Surface testing; Antibiotic susceptibility tests; Antibiotic treatment; Bacteria concentrations; Bacteria detection; Bacteria enrichment; Clinical diagnosis; Molecular detection; Surface enhanced Raman Scattering (SERS); Surface scattering |
顯示於: | 電機工程學系 |
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