https://scholars.lib.ntu.edu.tw/handle/123456789/561109
標題: | Diagnostic performance of whole-blood interferon-γ assay and enzyme-linked immunospot assay for active tuberculosis, | 作者: | Lai C.-C. Tan C.-K. Lin S.-H. Liao C.-H. YU-TSUNG HUANG PO-REN HSUEH |
公開日期: | 2011 | 卷: | 71 | 期: | 2 | 起(迄)頁: | 139-143 | 來源出版物: | Diagnostic Microbiology and Infectious Disease | 摘要: | The aim of this study was to compare the diagnostic performance of 2 interferon-γ release assays, an enzyme-linked immunospot assay (T-SPOT.TB; Oxford Immunotec Ltd., Oxford, UK) and the QuantiFERON-TB Gold in-Tube assay (QFT-GIT; Cellestis Ltd., Carnegie, Australia), in patients with suspected active tuberculosis (TB). From October 2009 to October 2011, a total of 200 patients with suspected TB were enrolled. Clinical and microbiological characteristics of the patients were collected and blood samples were obtained for T-SPOT.TB and QFT-GIT assays. Among the 200 subjects, 98 (49%) had culture-confirmed TB, 18 (9%) had probable TB, and the remaining 84 (42%) subjects did not have TB. The sensitivity, specificity, positive predictive value, and negative predictive value for active TB diagnosis by the T SPOT. TB were 83%, 71%, 81%, and 75%, respectively. For QFT-GIT, the sensitivity, specificity, positive predictive value, and negative predictive value for active TB diagnosis were 66%, 76%, 80%, and 62%, respectively. The QFT-GIT assay resulted in more indeterminate and false-negative results than the T-SPOT.TB assay, especially in immunocompromised patients. In conclusion, T-SPOT.TB had a higher sensitivity and resulted in fewer indeterminate results than the QFT-GIT assay for diagnosing active TB. ? 2011 Elsevier Inc. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/561109 | ISSN: | 0732-8893 | DOI: | 10.1016/j.diagmicrobio.2011.05.013 | SDG/關鍵字: | gamma interferon; adult; article; bacterium culture; blood sampling; clinical feature; controlled clinical trial; controlled study; diagnostic test accuracy study; diagnostic value; enzyme linked immunospot assay; extrapulmonary tuberculosis; false negative result; female; histopathology; human; human tissue; immunocompromised patient; interferon gamma release assay; lung biopsy; major clinical study; male; miliary tuberculosis; predictive value; priority journal; prospective study; sensitivity and specificity; tuberculosis; Adult; Aged; Enzyme-Linked Immunospot Assay; Female; Humans; Interferon-gamma; Interferon-gamma Release Tests; Male; Middle Aged; Mycobacterium tuberculosis; Predictive Value of Tests; Prospective Studies; Reagent Kits, Diagnostic; Taiwan; Tuberculosis |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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