Infections caused by Gordonia species at a medical centre in Taiwan, 1997 to 2008
Journal
Clinical Microbiology and Infection
Journal Volume
16
Journal Issue
9
Pages
1448-1453
Date Issued
2010
Author(s)
Abstract
The inability of conventional identification systems to accurately identify Gordonia spp. often results in the misdiagnosis of infections by these rare pathogens, which require genomic sequencing for precise identification. In the present study, we describe nine cases of the various types of infection caused by Gordonia spp. From 1997 to 2008, 66 isolates (from 30 patients) initially identified as Rhodoccus spp. by conventional biochemical methods, by the Bacteriology Laboratory of National Taiwan University Hospital, were retrospectively analysed to assess the accuracy of species identification. Fifteen of these isolates (from nine patients) were later found to be Gordonia spp. by two molecular methods: PCR-restriction fragment length polymorphism for heat shock protein gene (hsp65) and the 16S rRNA gene sequencing analysis. Gordonia sputi (n = 8) was the most common species, followed by Gordonia terrae (n = 7). Most of the isolates were isolated from blood (n = 11), followed by soft tissue (n = 2) and eye (n = 2). Five patients presented with bacteraemia and two of these had catheter-related bloodstream infection. Two patients had soft tissue infections and another two patients had infective keratitis and conjunctivitis. The random amplified polymorphic DNA patterns for isolates from different patients were different, indicating that they were genetically unrelated. Accurate identification with molecular methods is required if the role of Gordonia spp. in causing infection is to be recognized. ? 2010 The Authors. Journal Compilation ? 2010 European Society of Clinical Microbiology and Infectious Diseases.
SDGs
Other Subjects
amikacin; amoxicillin plus clavulanic acid; ampicillin; antibiotic agent; bacterial DNA; cefoxitin; chloramphenicol; ciprofloxacin; clindamycin; cotrimoxazole; dalfopristin plus quinupristin; erythromycin; genomic DNA; gentamicin; heat shock protein 65; imipenem; levofloxacin; linezolid; moxifloxacin; nitrofurantoin; oxacillin; penicillin G; rifampicin; RNA 16S; streptomycin; sulfamethoxazole; teicoplanin; tetracycline; unindexed drug; vancomycin; accuracy; Actinomycetales infection; adolescent; adult; aged; antibiotic sensitivity; article; bacteremia; bacterium identification; bacterium isolate; blood culture; catheter infection; catheter removal; cellulitis; clinical article; conjunctivitis; controlled study; debridement; female; Gordonia; Gordonia sputi; Gordonia terrae; human; keratitis; male; minimum inhibitory concentration; newborn; nonhuman; nucleotide sequence; polymerase chain reaction; priority journal; random amplified polymorphic DNA; restriction fragment length polymorphism; retrospective study; Rhodococcus; sequence analysis; soft tissue infection; Taiwan; wound infection; Gordonia sputi; Gordonia terrae
Type
journal article