|Title:||Green tea extract induces genes related to browning of white adipose tissue and limits weight-gain in high energy diet-fed rat||Authors:||Li-Han Chen
|Keywords:||Anti-obesity; Beige adipose tissue; Browning of white adipose tissue; Green tea extract; Pathways of browning||Issue Date:||2017||Publisher:||Swedish Nutrition Foundation||Journal Volume:||61||Journal Issue:||1||Start page/Pages:||1347480||Source:||Food & Nutrition Research||Abstract:||
Background: A wealth of research has reported on the anti-obesity effects of green tea extract (GTE). Although browning of white adipose tissue (WAT) has been reported to attenuate obesity, no study has disclosed the effects of GTE on browning in Sprague Dawley rats. Objectives: The aims of the study were to investigate the effects of GTE on anti-obesity and browning, and their underlying mechanisms. Methods: Four groups of rats (n=10/group) were used including a normal diet with vehicle treatment, and a high-energy diet (HED) with vehicle or GTE by oral gavage at 77.5 or 155 mg/kg/ day for 8 weeks. Body weight, fat accumulation, and serum biochemical parameters were used to evaluate obesity. The gene expressions were analyzed using RT-qPCR and western blotting. Results: GTE modulated HED-induced body weight, fat accumulation, and serum levels of triacylglycerol, total cholesterol, low-density lipoprotein, free fatty acids, aspartate aminotransferase, and alanine aminotransferase. Moreover, GTE enhanced the serum high-density lipoprotein. Most importantly, the biomarkers of beige adipose tissue were up-regulated in WAT in GTE-given groups. GTE induced genes involved in different pathways of browning, and reduced transducin-like enhancer protein-3 in WAT. Conclusion: Our results suggest that GTE may improve obesity through inducing browning in HED-fed rats. ? 2017 The Author(s).
|URI:||https://scholars.lib.ntu.edu.tw/handle/123456789/561628||ISSN:||1654-661X||DOI:||10.1080/16546628.2017.1347480||metadata.dc.subject.other:||alanine aminotransferase; aspartate aminotransferase; cholesterol; fatty acid; high density lipoprotein; low density lipoprotein; sinecatechins; transcription factor; transducin like enhancer protein 3; triacylglycerol; unclassified drug; alanine aminotransferase blood level; animal cell; animal experiment; animal model; animal tissue; antiobesity activity; Article; aspartate aminotransferase blood level; beige adipose tissue; body weight; body weight control; cholesterol blood level; controlled study; fatty acid blood level; gene expression; high calorie diet; high density lipoprotein cholesterol level; lipid storage; low density lipoprotein cholesterol level; male; nonhuman; obesity; rat; real time reverse transcription polymerase chain reaction; treatment duration; triacylglycerol blood level; upregulation; Western blotting; white adipose tissue
|Appears in Collections:||漁業科學研究所|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.