https://scholars.lib.ntu.edu.tw/handle/123456789/563743
標題: | Stromal cell-derived factor-1 increase αvβ3 integrin expression and invasion in human chondrosarcoma cells | 作者: | Lai T.-H. Fong Y.-C. WEN-MEI FU RONG-SEN YANG Tang C.-H. |
公開日期: | 2009 | 卷: | 218 | 期: | 2 | 起(迄)頁: | 334-342 | 來源出版物: | Journal of Cellular Physiology | 摘要: | Chondrosarcoma is a type of highly malignant tumor with a potent capacity to invade locally and cause distant metastasis. Chondrosarcoma shows a predilection for metastasis to the lungs. The stromal cell-derived factor 1 (SDF-1), constitutively secreted by human lung epithelium cells, has been shown to function in a key role for recruitment of neutrophils. Here, we found that human chondrosarcoma tissues and chondrosarcoma cell lines had significant expression of CXCR4 (SDF-1 receptor), which was higher than normal cartilage and human chondrocyte. SDF-1α and lung epithelium cells conditioned medium (LECM) induced the invasiveness of chondrosarcoma cells. SDF-1 siRNA inhibited LECM-induced invasion of chondrosarcoma cells and SDF-1α also directly induced the cell surface expression of αvβ3 but not α2β1 and α5β1 integrin. Activations of ERK and NF-κB pathways after SDF-1 treatment was demonstrated, and SDF-1α-induced expression of αvβ3 integrin and invasion activity was inhibited by the specific inhibitor and mutant of ERK and NF-κB cascades. Taken together, our results indicate that lung derived-SDF-1α enhances the invasiveness of chondrosarcoma cells by increasing αvβ3 integrin expression through the CXCR4/ERK/NF-κB signal transduction pathway. ? 2008 Wiley-Liss, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-58149243217&doi=10.1002%2fjcp.21601&partnerID=40&md5=c6dec70485061b11abb136839779e13f https://scholars.lib.ntu.edu.tw/handle/123456789/563743 |
ISSN: | 0021-9541 | DOI: | 10.1002/jcp.21601 | SDG/關鍵字: | chemokine receptor CXCR4; immunoglobulin enhancer binding protein; mitogen activated protein kinase; small interfering RNA; stromal cell derived factor 1; vitronectin receptor; stromal cell derived factor 1; article; cartilage cell; cell invasion; chondrosarcoma; controlled study; enzyme activation; human; human cell; lung alveolus epithelium; priority journal; protein expression; signal transduction; tumor cell; biological model; cancer invasion; drug effect; enzymology; gene expression regulation; genetics; metabolism; pathology; tumor cell line; upregulation; Cell Line, Tumor; Chemokine CXCL12; Chondrosarcoma; Extracellular Signal-Regulated MAP Kinases; Gene Expression Regulation, Neoplastic; Humans; Integrin alphaVbeta3; Models, Biological; Neoplasm Invasiveness; NF-kappa B; Receptors, CXCR4; Signal Transduction; Up-Regulation |
顯示於: | 醫學系 |
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