https://scholars.lib.ntu.edu.tw/handle/123456789/563983
標題: | Resveratrol enhances insulin secretion by blocking KATP and KV channels of beta cells | 作者: | WEN-PIN CHEN Chi T.-C. LEE-MING CHUANG MING-JAI SU |
公開日期: | 2007 | 卷: | 568 | 期: | 1月3日 | 起(迄)頁: | 269-277 | 來源出版物: | European Journal of Pharmacology | 摘要: | The present study investigated the effect of resveratrol on the electrophysiology and insulin secretion of pancreatic beta cells, and examined resveratrol-induced alterations in insulin levels and plasma glucose of normal and streptozotocin-induced diabetic rats. Whole-cell voltage clamp study in the MIN6 cell, a mouse β cell line, revealed that resveratrol significantly inhibited ATP-sensitive K+ current at 3?μmol/l, and voltage-gated K+ currents at 30?μmol/l. Ca2+-activated K+ current was activated by resveratrol at 100?μmol/l. In MIN6 cells stained with membrane potential dye DiBAC4(5), resveratrol markedly depolarized membrane potential at the concentrations of 3-100?μmol/l. Insulin secretion was increased in the presence of resveratrol in MIN6, Hit-T15, and RIN-m5F cells. Resveratrol (3?mg/kg, i.p.) increased insulin secretion associated with a lowering in plasma glucose in normal rats, but not in streptozotocin-diabetic rats within the initial 60?min. In conclusion, resveratrol can act as an insulin-secretagogue through IKATP and IKV inhibition which can contribute to plasma glucose lowering effect in normal rats. ? 2007 Elsevier B.V. All rights reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/563983 | ISSN: | 142999 | DOI: | 10.1016/j.ejphar.2007.04.062 | SDG/關鍵字: | adenosine triphosphate; adenosine triphosphate sensitive potassium channel; bis(1,3 dibutylbarbituric acid)pentamethine oxonol; calcium activated potassium channel; dye; glucose; insulin; potassium channel; resveratrol; streptozocin; unclassified drug; animal cell; animal experiment; animal model; article; concentration response; controlled study; drug mechanism; electrophysiology; glucose blood level; insulin blood level; insulin release; male; membrane depolarization; membrane potential; mouse; nonhuman; pancreas islet beta cell; potassium current; priority journal; rat; streptozocin diabetes; voltage clamp; Animals; Blood Glucose; Cell Line, Tumor; Cricetinae; Diabetes Mellitus, Experimental; Insulin; Insulin-Secreting Cells; Male; Membrane Potentials; Mice; Potassium Channel Blockers; Potassium Channels; Rats; Rats, Wistar; Stilbenes |
顯示於: | 藥理學科所 |
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