|Title:||Yuwen02f1 suppresses LPS-induced endotoxemia and adjuvant-induced arthritis primarily through blockade of ROS formation, NFkB and MAPK activation||Authors:||Hsu C.-C.
|Keywords:||Diphenylpyrazole; Inflammatory animal; MAPK; NFκB; ROS||Issue Date:||2013||Journal Volume:||85||Journal Issue:||3||Start page/Pages:||385-395||Source:||Biochemical Pharmacology||Abstract:||
Phagocytes release inflammatory mediators to defense harmful stimuli upon bacterial invasion, however, excessive inflammatory reaction leads to tissue damage and manifestation of pathological states. Therefore, targeting on uncontrolled inflammation seems feasible to control numerous inflammation-associated diseases. Under the drug screening process of synthetic diphenylpyrazole derivatives, we discovered compound yuwen02f1 possesses anti-inflammatory effects in decreasing the release of pro-inflammatory cytokines including TNFα and IL-6, nitric oxide, reactive oxygen species (ROS) as well as inhibiting migration of LPS-stimulated phagocytes. In addition, we observed that the molecular mechanism of yuwen02f1-mediated anti-inflammation is associated with decreasing phosphorylation of MAPK molecules including ERK1/2, JNK and p38, and attenuating translocation of p47phox and p67phox to the cell membrane. Yuwen02f1 also reverses IκBα degradation and attenuates the expression of NFκB-related downstream inducible enzymes like iNOS and COX-2. Furthermore, we found that yuwen02f1 attenuates some pathological syndromes of LPS-induced sepsis and adjuvant-induced arthritis in mice, as evidenced by decreasing the cytokine production, reversing thrombocytopenic syndrome, protecting the mice from tissue injury in septic mice, and attenuating paw edema in arthritic mice as well. These results suggest that yuwen02f1 is a potential anti-inflammatory agent for alleviating syndromes of acute and chronic inflammatory diseases as evidenced by attenuating the generation of cytokines and down-regulating the expression of iNOS and COX-2 through the blockade of ROS generation and NADPH oxidase, NFκB and MAPK activation pathways in LPS-stimulated phagocytes. ? 2012 Elsevier Inc. All rights reserved.
|URI:||https://scholars.lib.ntu.edu.tw/handle/123456789/564121||ISSN:||62952||DOI:||10.1016/j.bcp.2012.11.002||SDG/Keyword:||1 (4 fluorobenzyl) 5 (5 ethoxycarbonyl 2 furyl) 3 phenylpyrazole; antiinflammatory agent; cyclooxygenase 2; I kappa B alpha; immunoglobulin enhancer binding protein; inducible nitric oxide synthase; interleukin 6; lipopolysaccharide; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen activated protein kinase p38; nitric oxide; protein p47; protein p67; pyrazole derivative; reactive oxygen metabolite; stress activated protein kinase; tumor necrosis factor alpha; unclassified drug; yuwen 02f1; animal cell; animal experiment; animal model; animal tissue; antiinflammatory activity; arthritis; article; cell membrane; cell migration; controlled study; cytokine release; drug effect; endotoxemia; male; mouse; nonhuman; paw edema; phagocyte; priority journal; protein expression; protein phosphorylation; signal transduction; Animals; Anti-Inflammatory Agents; Arthritis, Experimental; Cell Line; Cell Movement; Cell Survival; Cyclooxygenase 2; Endotoxemia; Furans; Gene Expression Regulation; Humans; L-Lactate Dehydrogenase; Lipoproteins; Macrophages; Male; Mice; Mice, Inbred ICR; Mitogen-Activated Protein Kinases; Molecular Structure; Monocytes; NF-kappa B; Nitric Oxide; Nitric Oxide Synthase Type II; Pyrazoles; Reactive Oxygen Species
|Appears in Collections:||藥理學科所|
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