|Title:||Antiangiogenic mechanisms of PJ-8, a novel inhibitor of vascular endothelial growth factor receptor signaling||Authors:||Huang S.-W.
|Issue Date:||2012||Journal Volume:||33||Journal Issue:||5||Start page/Pages:||1022-1030||Source:||Carcinogenesis||Abstract:||
Angiogenesis occurs not only during tissue growth and development but also during wound healing and tumor progression. Angiogenesis is a balanced process controlled by proangiogenic and antiangiogenic molecules. As a critical factor in the induction of angiogenesis, vascular endothelial growth factor (VEGF) has become an attractive target for antiangiogenic and cancer therapeutic agents. In an effort to develop novel inhibitors to block VEGF signaling, we selected Pj-8, a benzimidazole derivative, and investigated its inhibitory mechanisms in human umbilical vascular endothelial cells (HUVECs). Pj-8 concentration-dependently inhibited VEGF-induced proliferation, migration and tube formation of HUVECs. Pj-8 also suppressed VEGF-induced microvessel sprouting from aortic rings ex vivo and suppressed neovascularization of implanted matrigel plugs in vivo. Pj-8 inhibited VEGF-induced phosphorylation of VEGF receptor (VEGFR) 2 and the downstream protein kinases, including Akt, focal adhesion kinase, extracellular signal-regulated kinases and Src. Results from in vitro kinase assay further demonstrated that Pj-8 suppressed the kinase activity of 3-phosphoinositide-dependent kinase 1 (PDK1). Using xenograft tumor angiogenesis model, Pj-8 markedly eliminated tumor-associated angiogenesis. Taken together, our findings suggest that Pj-8 inhibits VEGF and tumor cells MDA-MB-231-induced angiogenesis, and it may be a potential drug candidate in anticancer therapy. Downregulation of VEGFR2-mediated signaling may contribute to its antiangiogenic actions. ? The Author 2012. Published by Oxford University Press. All rights reserved.
|URI:||https://scholars.lib.ntu.edu.tw/handle/123456789/564123||ISSN:||1433334||DOI:||10.1093/carcin/bgs127||SDG/Keyword:||5 benzoyl 2 (5 methyl 2 furyl) 1h benzimidazole; benzimidazole derivative; mitogen activated protein kinase; phosphoinositide dependent protein kinase 1; pj 8; protein kinase B; unclassified drug; vasculotropin receptor; angiogenesis; antiangiogenic activity; article; cancer cell; cell migration; cell proliferation; female; human; human cell; in vitro study; in vivo culture; priority journal; protein phosphorylation; xenograft
|Appears in Collections:||藥理學科所|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.