https://scholars.lib.ntu.edu.tw/handle/123456789/565210
標題: | Using the Matrix-Induced Ion Suppression Method for Concentration Normalization in Cellular Metabolomics Studies | 作者: | GUAN-YUAN CHEN Liao H.-W. Tsai I.-L. Tseng Y.J. CHING-HUA KUO |
公開日期: | 2015 | 卷: | 87 | 期: | 19 | 起(迄)頁: | 9731-9739 | 來源出版物: | Analytical Chemistry | 摘要: | Studies of the cell metabolome greatly improve our understanding of cell biology. Currently, most cellular metabolomics studies control only cell numbers or protein content without adjusting the total metabolite concentration, mainly because of the lack of an effective concentration normalization method for cell metabolites. This study proposed a matrix-induced ion suppression (MIIS) method to measure the total amount of cellular metabolites by utilizing flow injection analysis coupled with electrospray ionization mass spectrometry (FIA-ESI-MS).We used series dilutions of HL-60 cell extracts to establish the relationship between cellular metabolite concentration and the degree of ion suppression of the ion suppression indicator, and a good correlation was obtained between 2- and 12-fold dilutions of cell extracts (R2 = 0.999). Two lung cancer cells, CL1-0 and CL1-5, were selected as the model cell lines to evaluate the efficacy of the MIIS method and the importance of metabolite concentration normalization. Through MIIS analysis, CL1-0 cells were found to contain metabolites at a concentration 2.1 times higher than in CL1-5, and the metastatic properties of CL1-5 could only be observed after 2.1-fold dilution of CL1-0 before metabolomic analysis. Our results demonstrated that the MIIS method is an effective approach for metabolite concentration normalization and that controlling metabolite concentrations can improve data integrity in cellular metabolomics studies. ? 2015 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84943166895&doi=10.1021%2facs.analchem.5b01869&partnerID=40&md5=0bdb919a953d8c59547b2dfa2081c3ec https://scholars.lib.ntu.edu.tw/handle/123456789/565210 |
ISSN: | 32700 | DOI: | 10.1021/acs.analchem.5b01869 | SDG/關鍵字: | Biomolecules; Cell culture; Cells; Electrodeposition; Electrospray ionization; Enzyme inhibition; Ions; Mass spectrometry; Matrix algebra; Metabolites; Cellular metabolites; Concentration normalizations; Effective approaches; Effective concentration; Electrospray ionization mass spectrometry; Flow injection analysis; Metabolite concentrations; Metabolomic analysis; Cytology; ion; adenocarcinoma; chemistry; electrospray mass spectrometry; flow injection analysis; human; lung; lung tumor; metabolism; metabolome; metabolomics; procedures; tumor cell line; Adenocarcinoma; Cell Line, Tumor; Flow Injection Analysis; Humans; Ions; Lung; Lung Neoplasms; Metabolome; Metabolomics; Spectrometry, Mass, Electrospray Ionization |
顯示於: | 藥學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。