Screening and confirmation of 62 drugs of abuse and metabolites in urine by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry
Journal
Journal of Analytical Toxicology
Journal Volume
37
Journal Issue
9
Pages
642-651
Date Issued
2013
Author(s)
Abstract
An ultra-high-performance liquid chromatography-quadrupole timeof-flight mass spectrometry (UHPLC-QTOF-MS) method for the screening and confirmation of 62 drugs of abuse and their metabolites in urine was developed in this study. The most commonly abused drugs, including amphetamines, opioids, cocaine, benzodiazepines (BZDs) and barbiturates, and many other new and emerging abused drugs, were selected as the analytes for this study. Urine samples were diluted 5-fold with deionized water before analysis. Using a superficially porous micro-particulate column and an acetic acid-based mobile phase, 54 basic and 8 acidic analytes could be detected within 15 and 12 min in positive and negative ionization modes, respectively. The MS collision energies for the 62 analytes were optimized, and their respective fragmentation patterns were constructed in the in-house library for confirmatory analysis. The coefficients of variation of the intra- and inter-day precision of the analyte responses all were <17.39%. All analytes, except barbital, showed matrix effects of 77-121%. The limits of detection of the 62 analytes were between 2.8 and 187.5 ng/mL, which were lower than their respective cut-off concentrations (20-500 ng/mL). Ten urine samples from patients undergoing methadone treatment were analyzed by the developed UHPLC-QTOF-MS method, and the results were compared with the immunoassay method. ? The Author [2013]. Published by Oxford University Press. All rights reserved.
SDGs
Other Subjects
3,4 methylenedioxyamphetamine; 3,4 methylenedioxymethamphetamine; 4 methoxyamphetamine; aminorex; amphetamine derivative; barbituric acid derivative; benzodiazepine derivative; butorphanol; cocaine; codeine; diamorphine; dihydrocodeine; ephedrine; fentanyl; ketamine; meprobamate; methadone; methamphetamine; methylephedrine; methylphenidate; morphine; nalorphine; norephedrine; pentazocine; pethidine; phentermine; pseudoephedrine; tramadol; unindexed drug; zolpidem; article; clinical article; concentration (parameters); drug abuse; drug metabolism; drug screening; female; human; immunoassay; ionization; limit of detection; male; methadone treatment; normal human; quadrupole mass spectrometry; time of flight mass spectrometry; ultra performance liquid chromatography; urinalysis; Algorithms; Autoanalysis; Chromatography, High Pressure Liquid; Heroin Dependence; Humans; Immunoassay; Indicators and Reagents; Limit of Detection; Mass Spectrometry; Methadone; Narcotics; Reference Standards; Reproducibility of Results; Street Drugs; Substance Abuse Detection
Type
journal article
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