https://scholars.lib.ntu.edu.tw/handle/123456789/565635
Title: | Gut Microbial Transcytosis Induced by Tumour Necrosis Factor-like 1A-dependent Activation of a Myosin Light Chain Kinase Splice Variant Contributes to Inflammatory Bowel Disease | Authors: | Pai, Yu-Chen Weng, Li-Ting SHU-CHEN WEI Wu, Li-Ling Shih, David Q Targan, Stephen R Turner, Jerrold R LINDA CHIA-HUI YU |
Issue Date: | 2021 | Publisher: | Oxford University Press | Journal Volume: | 15 | Journal Issue: | 2 | Start page/Pages: | 258-272 | Source: | Journal of Crohn's and Colitis | Abstract: | Background: Inflammatory bowel disease [IBD] is characterised by abnormal host-microbe interactions. Proinflammatory cytokine IFNγand a novel tumour necrosis factor [TNF] superfamily member, TL1A, have been implicated in epithelial barrier dysfunction. The divergent regulatory mechanisms of transcellular versus paracellular hyperpermeability remain poorly understood. Intestinal epithelia express two splice variants of long myosin light chain kinase [MLCK], of which the full-length MLCK1 differ from the shorter isoform MLCK2 by an Src kinase phosphorylation site. The aim of this study was to investigate the roles of MLCK splice variants in gut barrier defects under proinflammatory stress. Methods and Results: Upregulated expression of TL1A, IFNγ, and two MLCK variants was observed in human IBD biopsy specimens. The presence of intraepithelial bacteria preceded tight junction [TJ] damage in dextran sodium sulphate-treated and TL1A-transgenic mouse models. Lack of barrier defects was observed in long MLCK[-/-] mice. TL1A induced MLCK-dependent terminal web [TW] contraction, brush border fanning, and transepithelial bacterial internalisation. The bacterial taxa identified in the inflamed colonocytes included Escherichia, Enterococcus, Staphylococcus, and Lactobacillus. Recombinant TL1A and IFNγat low doses induced PI3K/Akt/MLCK2-dependent bacterial endocytosis, whereas high-dose IFNγcaused TJ opening via the iNOS/Src/MLCK1 axis. Bacterial internalisation was recapitulated in MLCK-knockout cells individually expressing MLCK2 but not MLCK1. Immunostaining showed different subcellular sites of phosphorylated MLC localised to the TJ and TW in the MLCK1- and MLCK2-expressing cells, respectively. Conclusions: Proinflammatory cytokines induced bacterial influx through transcellular and paracellular routes via divergent pathways orchestrated by distinct MLCK isoforms. Bacterial transcytosis induced by TL1A may be an alternative route causing symptom flares in IBD. ? 2020 The Author(s). All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85098993983&doi=10.1093%2fecco-jcc%2fjjaa165&partnerID=40&md5=cfb9dc8819a4c7c3e81538ae218dde57 https://scholars.lib.ntu.edu.tw/handle/123456789/565635 |
ISSN: | 1873-9946 | DOI: | 10.1093/ecco-jcc/jjaa165 | SDG/Keyword: | dextran sulfate; gamma interferon; inducible nitric oxide synthase; myosin light chain kinase; myosin light chain kinase 1; myosin light chain kinase 2; phosphatidylinositol 3 kinase; protein kinase B; protein tyrosine kinase; tumor necrosis factor; tumor necrosis factor like 1A; unclassified drug; adult; aged; animal cell; animal experiment; animal model; animal tissue; Article; clinical article; colon biopsy; colon cell; controlled study; Crohn disease; endocytosis; Enterococcus; enzyme activation; Escherichia; female; human; human tissue; intestine flora; Lactobacillus; male; mouse; nonhuman; pathogenesis; protein expression; Staphylococcus; tight junction; transcytosis; ulcerative colitis; upregulation |
Appears in Collections: | 醫學系 |
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