https://scholars.lib.ntu.edu.tw/handle/123456789/566464
標題: | Molecular Epidemiology and Phylodynamics of the Human Respiratory Syncytial Virus Fusion Protein in Northern Taiwan | 作者: | Chi H. Liu H.-F. Weng L.-C. Wang N.-Y. Chiu N.-C. Lai M.-J. Lin Y.-C. Chiu Y.-Y. Hsieh W.-S. LI-MIN HUANG |
公開日期: | 2013 | 卷: | 8 | 期: | 5 | 起(迄)頁: | e64012 | 來源出版物: | PLoS ONE | 摘要: | Background and Aims:The glycoprotein (G protein) and fusion protein (F protein) of respiratory syncytial virus (RSV) both show genetic variability, but few studies have examined the F protein gene. This study aimed to characterize the molecular epidemiology and phylodynamics of the F protein gene in clinical RSV strains isolated in northern Taiwan from 2000-2011.Methods:RSV isolates from children presenting with acute respiratory symptoms between July 2000 and June 2011 were typed based on F protein gene sequences. Phylogeny construction and evaluation were performed using the neighbor-joining (NJ) and maximum likelihood (ML) methods. Phylodynamic patterns in RSV F protein genes were analyzed using the Bayesian Markov Chain Monte Carlo framework. Selection pressure on the F protein gene was detected using the Datamonkey website interface.Results:From a total of 325 clinical RSV strains studied, phylogenetic analysis showed that 83 subgroup A strains (RSV-A) could be further divided into three clusters, whereas 58 subgroup B strains (RSV-B) had no significant clustering. Three amino acids were observed to differ between RSV-A and -B (positions 111, 113, and 114) in CTL HLA-B*57- and HLA-A*01-restricted epitopes. One positive selection site was observed in RSV-B, while none was observed in RSV-A. The evolution rate of the virus had very little change before 2000, then slowed down between 2000 and 2005, and evolved significantly faster after 2005. The dominant subtypes of RSV-A in each epidemic were replaced by different subtypes in the subsequent epidemic.Conclusions:Before 2004, RSV-A infections were involved in several small epidemics and only very limited numbers of strains evolved and re-emerged in subsequent years. After 2005, the circulating RSV-A strains were different from those of the previous years and continued evolving through 2010. Phylodynamic pattern showed the evolutionary divergence of RSV increased significantly in the recent 5 years in northern Taiwan. ? 2013 Chi et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878464992&doi=10.1371%2fjournal.pone.0064012&partnerID=40&md5=3d53e94c940697094da5fc36b75385be https://scholars.lib.ntu.edu.tw/handle/123456789/566464 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0064012 | SDG/關鍵字: | amino acid; epitope; virus fusion protein; virus RNA; acute respiratory tract disease; article; Bayes theorem; child; controlled study; cytotoxic T lymphocyte; epidemic; evolution; F protein gene; female; gene sequence; human; infant; lower respiratory tract infection; major clinical study; male; molecular epidemiology; molecular evolution; Monte Carlo method; nonhuman; nucleotide sequence; phylodynamics; phylogeny; preschool child; Respiratory syncytial pneumovirus; reverse transcription polymerase chain reaction; school child; sequence analysis; Taiwan; virus gene; virus isolation; virus strain; Bayes Theorem; Cell Line; Cell Line, Tumor; Child; Child, Preschool; Epitopes, T-Lymphocyte; Evolution, Molecular; Female; Genetic Variation; Hep G2 Cells; HLA-A1 Antigen; HLA-B Antigens; Humans; Infant; Infant, Newborn; Male; Molecular Epidemiology; Molecular Sequence Data; Monte Carlo Method; Phylogeny; Respiratory Syncytial Virus Infections; Respiratory Syncytial Virus, Human; T-Lymphocytes, Cytotoxic; Taiwan; Viral Fusion Proteins; Human respiratory syncytial virus; Respiratory syncytial virus; Rice stripe virus; Subgroup A; Subgroup B |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。