https://scholars.lib.ntu.edu.tw/handle/123456789/567511
標題: | IL-17 contributes to cell-mediated defense against pulmonary Yersinia pestis infection | 作者: | JR-SHIUAN LIN Kummer, Lawrence W Szaba, Frank M Smiley, Stephen T |
公開日期: | 1-二月-2011 | 卷: | 186 | 期: | 3 | 來源出版物: | Journal of immunology (Baltimore, Md. : 1950) | 摘要: | Pneumonic plague is one of the world's most deadly infectious diseases. The causative bacterium, Yersinia pestis, has the potential to be exploited as a biological weapon, and no vaccine is available. Vaccinating B cell-deficient mice with D27-pLpxL, a live attenuated Y. pestis strain, induces cell-mediated protection against lethal pulmonary Y. pestis challenge. In this article, we demonstrate that prime/boost vaccination with D27-pLpxL confers better protection than prime-only vaccination. The improved survival does not result from enhanced bacterial clearance but is associated with increased levels of IL-17 mRNA and protein in the lungs of challenged mice. The boost also increases pulmonary numbers of IL-17-producing CD4 T cells. Interestingly, most of these cells simultaneously produce canonical type 1 and type 17 cytokines; most produce IL-17 and TNF-α, and many produce IL-17, TNF-α, and IFN-γ. Neutralizing IL-17 counteracts the improved survival associated with prime/boost vaccination without significantly impacting bacterial burden. Thus, IL-17 appears to mediate the enhanced protection conferred by booster immunization. Although neutralizing IL-17 significantly reduces neutrophil recruitment to the lungs of mice challenged with Y. pestis, this impact is equally evident in mice that receive one or two immunizations with D27-pLpxL, suggesting it cannot suffice to account for the improved survival that results from booster immunization. We conclude that IL-17 plays a yet to be identified role in host defense that enhances protection against pulmonary Y. pestis challenge, and we suggest that pneumonic plague vaccines should aim to induce mixed type 1 and type 17 cellular responses. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/567511 | ISSN: | 00221767 | DOI: | 10.4049/jimmunol.1003303 |
顯示於: | 免疫學研究所 |
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