https://scholars.lib.ntu.edu.tw/handle/123456789/567813
標題: | Evaluation of the stability of enterovirus 71 virus-like particle | 作者: | Lin S.-Y. Chung Y.-C. Chiu H.-Y. Chi W.-K. BOR-LUEN CHIANG Hu Y.-C. |
關鍵字: | Baculovirus; Enterovirus 71; Insect cell; Stability; Virus-like particle | 公開日期: | 2014 | 卷: | 117 | 期: | 3 | 起(迄)頁: | 366-371 | 來源出版物: | Journal of Bioscience and Bioengineering | 摘要: | Enterovirus 71 (EV71) is responsible for the outbreaks of hand-foot-and-mouth disease that caused significant mortality in children, but no vaccine is available yet. EV71 virus-like particle (VLP) is the empty capsid consisting of viral structural proteins but can elicit potent immune responses, rendering VLP a promising EV71 vaccine candidate. To evaluate whether VLP remains stable after long-term storage, which is crucial for advancing the VLP vaccine to the clinical setting, we evaluated the effects of NaCl concentration, buffers and temperatures on the VLP stability. We first validated the use of dynamic light scattering (DLS) for measuring the hydrodynamic diameter (?30-35nm) of VLP, which was close to the VLP diameter (?25-27nm) as measured by transmission electron microscopy (TEM). Using these techniques, we found that EV71 VLP remained stable for 5 months in sodium phosphate (NaPi) buffers with various NaCl concentrations. EV71 VLP also remained morphologically stable in NaPi, citrate and TE+ buffers for 5 months, yet the enzyme-linked immunosorbent assay (ELISA) revealed that the VLP stored in citrate and TE+ buffers partially lost the immunogenicity after 5 months. In contrast, the VLP stored in the NaPi buffer at 4°C remained stable macroscopically and microscopically for 5 months, as judged from the DLS, TEM and ELISA. The VLP stored at 25°C and 37°C also retained stability for 1 month, which would obviate the need of a cold chain during the shipping. These data altogether proved the stability of EV71 VLP and suggested that the VLP is amenable to bioprocessing and storage. ? 2013 The Society for Biotechnology, Japan. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84893798501&doi=10.1016%2fj.jbiosc.2013.08.015&partnerID=40&md5=0359d37f31af76c51fdee22043693bd3 https://scholars.lib.ntu.edu.tw/handle/123456789/567813 |
ISSN: | 1389-1723 | DOI: | 10.1016/j.jbiosc.2013.08.015 | SDG/關鍵字: | Baculovirus; Enterovirus 71; Insect cell; Stability; Virus-like particle; Animals; Antibodies, Viral; Cell Line; Enterovirus; Enzyme-Linked Immunosorbent Assay; Insects; Microscopy, Electron, Transmission; Particle Size; Protein Stability; Sodium Chloride; Vaccines, Virus-Like Particle; Viral Structural Proteins; Viral Vaccines |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。