https://scholars.lib.ntu.edu.tw/handle/123456789/567870
標題: | Detection of circulating endothelial cells via a microfluidic disk | 作者: | Chen, K.-C. Lee, T.-P. Pan, Y.-C. Chiang, C.-L. Chen, C.-L. YAO-HSU YANG BOR-LUEN CHIANG Lee, H. ANDREW WO |
公開日期: | 2011 | 卷: | 57 | 期: | 4 | 起(迄)頁: | 586-592 | 來源出版物: | Clinical Chemistry | 摘要: | BACKGROUND: Circulating endothelial cells (CECs) in the blood are rare but have been shown to be associated with various diseases. With the ratio of CECs to peripheral blood mononuclear cells (PBMCs) less than 1 part per thousand, their separation from PBMCs and detection are challenging. We present a means of detecting CECs from PBMCs via an economical microfluidic disk with a model cell system [human umbilical vein endothelial cells (HUVECs) in PBMCs], along with demonstration of its efficacy clinically. METHODS: To enrich these rare cells, we used immunomagnetic beads and a tailor-made magnet on the disk. CEC-simulating HUVECs, as target cells, were stained with primary anti-CD146-phycoerythrin antibody and bound with secondary antibody on antiphycoerythrin magnetic beads. PBMCs served as nontarget cells and were labeled with anti-CD45-FITC antibody. RESULTS: When hundreds of HUVECs were mixed in 106 PBMCs, 95% of spiked HUVECs were detected. This yield also held for 60 HUVEC in <104 PBMCs. We compared data from flow cytometry with that from the disk: CEC counts in 50 μL blood from patients with systemic lupus erythematosus were 61.1 (21.5), significantly higher (P < 0.01) than those of healthy donors, 31.2 (13.3). CONCLUSIONS: The count of CECs is a suitable marker for symptoms of systemic lupus erythematosus. The microfluidic disk system should be a viable platform for detection of CECs. ? 2011 American Association for Clinical Chemistry. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79956068774&doi=10.1373%2fclinchem.2010.157404&partnerID=40&md5=799ed8f4fe9bd124c09939ee829f4423 https://scholars.lib.ntu.edu.tw/handle/123456789/567870 |
ISSN: | 0009-9147 | DOI: | 10.1373/clinchem.2010.157404 | SDG/關鍵字: | antibody; CD146 antibody; CD45 antibody; cell antibody; fluorescein isothiocyanate; phycoerythrin; unclassified drug; antibody labeling; article; blood analysis; blood sampling; cell count; cell function; cell separation; circulating endothelial cell; circulation; controlled study; disk diffusion; endothelium cell; flow cytometry; human; human cell; immunomagnetic separation; magnet; magnetism; microfluidic disk; microfluidics; peripheral blood mononuclear cell; systemic lupus erythematosus; target cell; umbilical vein endothelial cell; Endothelium, Vascular; Flow Cytometry; Humans; Microfluidics |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。