Application of interleukin-12 expressing dendritic cells for the treatment of animal model of leukemia
Journal
Experimental Biology and Medicine
Journal Volume
234
Journal Issue
8
Pages
952-960
Date Issued
2009
Author(s)
Abstract
Residual cancer cells appearing in blood circulation reduce the effects of radiotherapy or chemotherapy in cancer patients. It has been well documented that cultured dendritic cells can be used as a powerful tool to induce immune response. In this study, we administered different manipulations of dendritic cells (DCs), including DCs pulsed with tumor cell lysate (TCL), transfected with adenoviral IL-12 vector (AdIL-12) and transfected with AdIL-12 after being pulsed with TCL, to determine whether improved DCs based immunotherapy can specifically suppress the metastasis of tumor cells. The results demonstrated that administration of engineered DCs that transfected with AdIL-12 after being pulsed with TCL to mice with leukemia had a better protective effect than that of DCs either pulsed with TCL or transfected with AdIL-12. Moreover, depletion of CD81 cells in the engineered DCs administered leukemia mice reduced the protective effect. These results suggest that DCs modified with TCL and AdIL-12 can prolong survival time by enhancing the activity of cytotoxic T cells. Although more studies on the mechanisms are needed, cytokine genes engineered DCs provide a promising therapeutic potential on the murine model of leukemia. Copyright ? 2009 by the Society for Experimental Biology and Medicine.
Subjects
Adenoviral vector; Dendritic cells; Interleukin-12; Leukemia; RL♂1
SDGs
Other Subjects
adenovirus vector; interleukin 12; animal cell; animal experiment; animal model; antineoplastic activity; article; cancer immunotherapy; CD8+ T lymphocyte; cellular immunity; controlled study; cytotoxic T lymphocyte; dendritic cell; gene expression; genetic transfection; leukemia; mouse; nonhuman; survival time; viral gene delivery system; Adenoviridae; Animals; Cell Line, Tumor; Cell Membrane; Dendritic Cells; Disease Models, Animal; Genetic Engineering; Immunity, Cellular; Interleukin-12; Leukemia; Mice; T-Lymphocytes, Cytotoxic; Transfection; Vaccination; Animalia; Murinae; Mus
Type
journal article