https://scholars.lib.ntu.edu.tw/handle/123456789/567910
標題: | Application of interleukin-12 expressing dendritic cells for the treatment of animal model of leukemia | 作者: | Tsai B.-Y. Lin Y.-L. BOR-LUEN CHIANG |
關鍵字: | Adenoviral vector; Dendritic cells; Interleukin-12; Leukemia; RL♂1 | 公開日期: | 2009 | 卷: | 234 | 期: | 8 | 起(迄)頁: | 952-960 | 來源出版物: | Experimental Biology and Medicine | 摘要: | Residual cancer cells appearing in blood circulation reduce the effects of radiotherapy or chemotherapy in cancer patients. It has been well documented that cultured dendritic cells can be used as a powerful tool to induce immune response. In this study, we administered different manipulations of dendritic cells (DCs), including DCs pulsed with tumor cell lysate (TCL), transfected with adenoviral IL-12 vector (AdIL-12) and transfected with AdIL-12 after being pulsed with TCL, to determine whether improved DCs based immunotherapy can specifically suppress the metastasis of tumor cells. The results demonstrated that administration of engineered DCs that transfected with AdIL-12 after being pulsed with TCL to mice with leukemia had a better protective effect than that of DCs either pulsed with TCL or transfected with AdIL-12. Moreover, depletion of CD81 cells in the engineered DCs administered leukemia mice reduced the protective effect. These results suggest that DCs modified with TCL and AdIL-12 can prolong survival time by enhancing the activity of cytotoxic T cells. Although more studies on the mechanisms are needed, cytokine genes engineered DCs provide a promising therapeutic potential on the murine model of leukemia. Copyright ? 2009 by the Society for Experimental Biology and Medicine. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-68149092683&doi=10.3181%2f0805-RM-165&partnerID=40&md5=755ce03b4982903b9c96f3837003adaa https://scholars.lib.ntu.edu.tw/handle/123456789/567910 |
ISSN: | 1535-3702 | DOI: | 10.3181/0805-RM-165 | SDG/關鍵字: | adenovirus vector; interleukin 12; animal cell; animal experiment; animal model; antineoplastic activity; article; cancer immunotherapy; CD8+ T lymphocyte; cellular immunity; controlled study; cytotoxic T lymphocyte; dendritic cell; gene expression; genetic transfection; leukemia; mouse; nonhuman; survival time; viral gene delivery system; Adenoviridae; Animals; Cell Line, Tumor; Cell Membrane; Dendritic Cells; Disease Models, Animal; Genetic Engineering; Immunity, Cellular; Interleukin-12; Leukemia; Mice; T-Lymphocytes, Cytotoxic; Transfection; Vaccination; Animalia; Murinae; Mus |
顯示於: | 醫學系 |
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