https://scholars.lib.ntu.edu.tw/handle/123456789/567973
標題: | Oral immunization of mice using transgenic tomato fruit expressing VP1 protein from enterovirus 71 | 作者: | Chen H.-F. Chang M.-H. BOR-LUEN CHIANG SHIH-TONG JENG |
公開日期: | 2006 | 卷: | 24 | 期: | 15 | 起(迄)頁: | 2944-2951 | 來源出版物: | Vaccine | 摘要: | Enterovirus 71 (EV71) causes seasonal epidemics of hand-foot-and-mouth disease associated with fatal neurological complications in young children, and several major outbreaks have occurred recently. This study developed an effective antiviral agent by transforming the gene for VP1 protein, a previously defined epitope and also a coat protein of EV71, into tomato plant. VP1 protein was first fused with sorting signals to enable it to be retained in the endoplasmic reticulum of tomato plant, and its expression level increased to 27 μg/g of fresh tomato fruit. Transgenic tomato fruit expressing VP1 protein was then used as an oral vaccine, and the development of VP1-specific fecal IgA and serum IgG were observed in BALB/c mice. Additionally, serum from mice fed transgenic tomato could neutralize the infection of EV71 to rhabdomyosarcoma cells, indicating that tomato fruit expressing VP1 was successful in orally immunizing mice. Moreover, the proliferation of spleen cells from orally immunized mice was stimulated by VP1 protein, and provided further evidence of both humoral and cellular immunity. Results of this study not only demonstrate the feasibility of using transgenic tomato as an oral vaccine to generate protective immunity in mice against EV71, but also suggest the probability of enterovirus vaccine development. ? 2005 Elsevier Ltd. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33644913110&doi=10.1016%2fj.vaccine.2005.12.047&partnerID=40&md5=6efe243e1831d3afc48c5adeed617352 https://scholars.lib.ntu.edu.tw/handle/123456789/567973 |
ISSN: | 0264-410X | DOI: | 10.1016/j.vaccine.2005.12.047 | SDG/關鍵字: | immunoglobulin A; immunoglobulin G; protein VP1; unclassified drug; virus vaccine; vp1 vaccine; animal experiment; article; cell proliferation; cell stimulation; cellular immunity; drug activity; drug mechanism; drug synthesis; endoplasmic reticulum; Enterovirus 71; female; gene expression; genetic transformation; humoral immunity; immune response; immunization; immunogenicity; immunoglobulin blood level; infection control; mouse; nonhuman; priority journal; protein expression; rhabdomyosarcoma; signal transduction; spleen cell; tomato; transgenic plant; Administration, Oral; Animals; Antibodies, Viral; Capsid Proteins; Cytokines; Enterovirus; Enzyme-Linked Immunosorbent Assay; Female; Lycopersicon esculentum; Lymphocytes; Mice; Mice, Inbred BALB C; Plants, Genetically Modified; Protein Sorting Signals; Spleen; Vaccines, Edible; Vaccines, Synthetic; Viral Vaccines |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。