https://scholars.lib.ntu.edu.tw/handle/123456789/567993
標題: | Polysaccharide purified from Ganoderma lucidum induced activation and maturation of human monocyte-derived dendritic cells by the NF-κB and p38 mitogen-activated protein kinase pathways | 作者: | Lin Y.-L. Liang Y.-C. Lee S.-S. BOR-LUEN CHIANG |
關鍵字: | IFN-γ; IL-10; IL-12; PS-G; Signal transduction; T cells | 公開日期: | 2005 | 卷: | 78 | 期: | 2 | 起(迄)頁: | 533-543 | 來源出版物: | Journal of Leukocyte Biology | 摘要: | Ganoderma lucidum, a fungus native to China, has been widely used to promote health and longevity in the Chinese. The polysaccharide component with a branched (1→6)-β-D-glucan moiety of G. lucidum (PS-G) has been reported to exert anti-tumor activity and activation of natural killer cells. In this study, we investigated the effects of PS-G on human monocyte-derived dendritic cells (DC). Treatment of DC with PS-G resulted in the enhanced cell-surface expression of CD80, CD86, CD83, CD40, CD54, and human leukocyte antigen (HLA)-DR, as well as the enhanced production of interleukin (IL)-12p70, p40, and IL-10 and also IL-12p35, p40, and IL-10 mRNA expression, and the capacity for endocytosis was suppressed in DC. In addition, treatment of DC with PS-G resulted in enhanced T cell-stimulatory capacity and increased T cell secretion of interferon-γ and IL-10. Neutralization with antibodies against Toll-like receptor (TLR)-4 inhibited the PS-G-induced production of IL-12 p40 and IL-10, suggesting a vital role for TLR-4 in signaling DC upon incubation with PS-G. Further study showed that PS-G was able to augment inhibitor of κB (IκB) kinase and nuclear factor (NF)-κB activity and also IκBα and p38 mitogen-activated protein kinase (MAPK) phosphorylation. Further, inhibition of NF-κB by helenalin and p38 MAPK by SB98059 prevented the effects of PS-G in the expression of CD80, CD86, CD83, CD40, CD54, and HLA-DR and production of IL-12p70, p40, and IL-10 in various degrees. Taken together, our data demonstrate that PS-G can effectively promote the activation and maturation of immature DC, suggesting that PS-G may possess a potential in regulating immune responses. ? Society for Leukocyte Biology. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-23044475016&doi=10.1189%2fjlb.0804481&partnerID=40&md5=2f83c22e5720d92e134eceefcbcf34ad https://scholars.lib.ntu.edu.tw/handle/123456789/567993 |
ISSN: | 0741-5400 | DOI: | 10.1189/jlb.0804481 | SDG/關鍵字: | 2 (2 amino 3 methoxyphenyl)chromone; 4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole; B7 antigen; beta 1,6 glucan; CD40 antigen; CD83 antigen; CD86 antigen; gamma interferon; helenalin; HLA DR antigen; I kappa B alpha; I kappa B kinase; immunoglobulin enhancer binding protein; intercellular adhesion molecule 1; interleukin 10; interleukin 12; interleukin 12p35; interleukin 12p40; interleukin 12p70; messenger RNA; mitogen activated protein kinase p38; polysaccharide; toll like receptor 4; unclassified drug; antigen expression; antineoplastic activity; article; cell activation; cell maturation; China; controlled study; cytokine production; cytokine release; dendritic cell; drug isolation; endocytosis; enzyme phosphorylation; Ganoderma lucidum; human; human cell; immune response; immunoreactivity; immunoregulation; natural killer cell; nonhuman; priority journal; T lymphocyte activation; Antibodies, Monoclonal; Antigens, CD; Cell Differentiation; Cells, Cultured; Dendritic Cells; HLA-DR Antigens; Humans; I-kappa B Proteins; Interleukins; MAP Kinase Signaling System; Membrane Glycoproteins; Monocytes; NF-kappa B; p38 Mitogen-Activated Protein Kinases; Polysaccharides; Receptors, Cell Surface; Reishi; Toll-Like Receptor 4; Toll-Like Receptors; Fungi; Ganoderma lucidum |
顯示於: | 醫學系 |
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