Effect of oxygen tension on stability and expression of a killer toxin chimeric plasmid in a chemostat culture of Saccharomyces cerevisiae
Journal
Applied Microbiology and Biotechnology
Journal Volume
27
Journal Issue
1
Pages
72-74
Date Issued
1987
Author(s)
Hassan H.M.
Abstract
The effects of oxygen tension and dilution rate on expression and stability of killer toxin chimeric plasmid (pADH-10A) in transformants of wine yeast (Montrachet 522) were investigated using a glucose-limited chemostat culture. Killer toxin activity increased as a function of dilution rate when transformants were grown in N2 or air, but not in 100% O2. Killer transformants grown in the presence of N2 had 2-4 fold and 10-15 fold higher toxin expression than that in the presence of air and 100% O2, respectively. Plasmid stability increased as a function of dilution rate, regardless of the oxygen tension used. However, transformants grown in air gave the highest plasmid stability. © 1987 Springer-Verlag.
SDGs
Publisher
Springer-Verlag
Type
journal article