https://scholars.lib.ntu.edu.tw/handle/123456789/568265
Title: | Cellular Assays for Measuring Dynamin Activity in Muscle Cells | Authors: | Laiman, Jessica YA-WEN LIU |
Keywords: | Bin1; Dynamin-2; GLUT4; Muscle disorder; T-Tubule | Issue Date: | 2020 | Journal Volume: | 2159 | Source: | Methods in molecular biology (Clifton, N.J.) | Abstract: | Dynamin is one of the best-studied membrane fission machineries, which mediates endocytic vesicle pinch-off from the plasma membrane. Among the three dynamin isoforms encoded in mammalian genome, dynamin-2 is the ubiquitously expressed isoform and leads to human muscular or neuronal diseases when mutants causing hyperactivity or hypoactivity of its membrane fission activity occur. While transferrin uptake is the most commonly used assay to measure dynamin activity in cultured cells, here we provide two different methods to quantitatively examine the activity of dynamin in myoblasts and myotubes, i.e., Bin1-tubule vesiculation and glucose transporter 4 fractionation assays, respectively. These methods could provide a quantitative measurement of dynamin activity in both differentiated and undifferentiated myoblasts. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/568265 | ISBN: | 978-1-0716-0675-9 978-1-0716-0676-6 |
ISSN: | 10643745 | DOI: | 10.1007/978-1-0716-0676-6_13 | SDG/Keyword: | dynamin; glucose transporter 4; biological marker; dynamin; glucose transporter 4; animal cell; C2C12 cell line; cell assay; cell differentiation; cell fractionation; cell membrane; cellular distribution; controlled study; enzyme activity; mouse; myoblast; myotube; nonhuman; protein analysis; quantitative analysis; animal; cell culture; cell line; confocal microscopy; enzyme activation; enzyme assay; fluorescent antibody technique; gene expression; genetic transfection; genetics; metabolism; muscle cell; myoblast; procedures; rat; Animals; Biomarkers; Cell Line; Cells, Cultured; Dynamins; Enzyme Activation; Enzyme Assays; Fluorescent Antibody Technique; Gene Expression; Glucose Transporter Type 4; Microscopy, Confocal; Muscle Cells; Myoblasts; Rats; Transfection |
Appears in Collections: | 分子醫學研究所 |
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