https://scholars.lib.ntu.edu.tw/handle/123456789/568412
標題: | Age-related immune clearance of hepatitis B virus infection requires the establishment of gut microbiota | 作者: | Chou H.-H. Chien W.-H. Wu L.-L. Cheng C.-H. Chung C.-H. Horng J.-H. YEN-HSUAN NI Tseng H.-T. Wu D. Lu X. HURNG-YI WANG PEI-JER CHEN Chen D.-S. |
公開日期: | 2015 | 出版社: | National Academy of Sciences | 卷: | 112 | 期: | 7 | 起(迄)頁: | 2175-2180 | 來源出版物: | Proceedings of the National Academy of Sciences of the United States of America | 摘要: | A unique feature of hepatitis B virus (HBV) infection in humans is that viral clearance heavily depends on the age of exposure. However, the reason for this remains unclear. Here we show that gut microbiota contribute to the age dependence of HBV immunity in a hydrodynamic transfection mouse model. Although adult (12-wkold) C3H/HeN mice cleared HBV within 6 wk postinjection (wpi), their young (6-wk-old) counterparts remained HBV-positive at 26 wpi. Sterilization of gut microbiota from 6 to 12 wk of age using antibiotics prevented adult mice from rapidly clearing HBV. Young mice with the Toll-like-receptor (TLR) 4 mutation (C3H/HeJ) exhibited rapid HBV clearance. The results suggest that an immuno-tolerating pathway to HBV prevailed in young mice, before the establishment of gut bacteria, through a TLR4-dependent pathway and that the maturation of gut microbiota in adult mice stimulated liver immunity, resulting in rapid HBV clearance. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983722767&doi=10.1073%2fpnas.1424775112&partnerID=40&md5=65878cf3ff3ee549ba8cbe5d9fb33437 https://scholars.lib.ntu.edu.tw/handle/123456789/568412 |
ISSN: | 0027-8424 | DOI: | 10.1073/pnas.1424775112 | SDG/關鍵字: | ampicillin; hepatitis B surface antigen; metronidazole; neomycin; toll like receptor; vancomycin; adaptive immunity; adult; age; animal cell; animal experiment; animal model; animal tissue; antibiotic therapy; antibody production; Article; cellular immunity; controlled study; enzyme linked immunospot assay; haplotype; hepatitis B; Hepatitis B virus; humoral immunity; hydrodynamics based transfection; innate immunity; intestine flora; mouse; nonhuman; Northern blotting; portal vein blood flow; priority journal; protein expression; real time polymerase chain reaction; RNA extraction; viral clearance; virus immunity; Western blotting; animal; Bagg albino mouse; hepatitis B; human; immunology; intestine; microbiology; microflora; nonobese diabetic mouse; Hepatitis B virus; Mus; Animals; Hepatitis B; Humans; Intestines; Mice; Mice, Inbred BALB C; Mice, Inbred NOD; Microbiota |
顯示於: | 臨床醫學研究所 |
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