https://scholars.lib.ntu.edu.tw/handle/123456789/568465
標題: | Antigenic analysis of divergent genotypes human Enterovirus 71 viruses by a panel of neutralizing monoclonal antibodies: Current genotyping of EV71 does not reflect their antigenicity | 作者: | Chen Y. Li C. He D. Cheng T. Ge S. Shih J.W.K. Zhao Q. PEI-JER CHEN Zhang J. Xia N. |
關鍵字: | Antigenicity; EV71; Monoclonal antibody; Neutralizing; Typing; Vaccine | 公開日期: | 2013 | 出版社: | Elsevier Ltd | 卷: | 31 | 期: | 2 | 起(迄)頁: | 425-430 | 來源出版物: | Vaccine | 摘要: | In recent year, Enterovirus 71 (EV71)-associated hand, foot and mouth disease (HFMD) has become an important public health issue in China. EV71 has been classified into genotypes A, B1-B5 and C1-C5. With such genetic diversity, whether the convalescent or recovery antibody responses can cross-protect infections from other genotypes remains a question. Understanding of the antigenicity of such diverse genetic EV71 isolates is crucial for the EV71 vaccine development. Here, a total of 186 clones anti-EV71 MAbs was generated and characterized with Western blot and cell-based neutralization assay. Forty neutralizing anti-EV71 MAbs were further used to analyze the antigenic properties of 18 recent EV71 isolates representing seven genotypes in neutralization assay. We found that most neutralizing anti-EV71 MAbs are specific to conformational epitopes. We also classified the 40 neutralizing anti-EV71 MAbs into two classes according to their reactivity patterns with 18 EV71 isolates. Class I MAb can neutralize all isolates, suggesting conserved epitopes are present among EV71. Class II MAb includes four subclasses (IIa-IId) and neutralizes only subgroups of EV71 strains. Conversely, 18 EV71 strains were grouped into antigenic types 1 and four antigenic subtypes (2.1-2.4). These results suggest that the current genotyping of EV71 does not reflect their antigenicity which may be important in the selection of EV71 vaccine strains. This panel of neutralizing anti-EV71 MAbs may be useful for the recognition of emerging antigenic variants of EV71 and vaccine development. ? 2012 Elsevier Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984570507&doi=10.1016%2fj.vaccine.2012.10.032&partnerID=40&md5=42cdbee6b57a478ce74b103cb02242e9 https://scholars.lib.ntu.edu.tw/handle/123456789/568465 |
ISSN: | 0264-410X | DOI: | 10.1016/j.vaccine.2012.10.032 | SDG/關鍵字: | epitope; monoclonal antibody; antigenicity; article; controlled study; embryo; Enterovirus 71; genotype; human; human cell; nonhuman; nucleotide sequence; priority journal; protein conformation; virus isolation; virus neutralization; Western blotting |
顯示於: | 臨床醫學研究所 |
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