https://scholars.lib.ntu.edu.tw/handle/123456789/568538
標題: | DNA detection using commercial mobile phones | 作者: | Lee D. Chou W.P. Shiou-Hwei Yeh PEI-JER CHEN Chen P.H. |
公開日期: | 2011 | 出版社: | Elsevier Ltd | 卷: | 26 | 期: | 11 | 起(迄)頁: | 4349-4354 | 來源出版物: | Biosensors and Bioelectronics | 摘要: | This study investigates the feasibility of using mobile phones cameras for DNA detection. DNA amplification uses the convective polymerase chain reaction (cPCR) technique due to its simple mechanism, which requires no thermal cycling control. Fluorescence increment analysis and information entropy analysis are employed separately to determine whether the test samples contain target DNA (Positive) or not (Negative). The fluorescence increment method uses the brightness of the captured images before and after DNA amplification to calculate Δ F Δ F values above a threshold level indicate that the test sample is positive. The information entropy method defines the probability, P(C/X), which indicates whether the fluorescence image tends towards a specific shape. If a DNA template is successfully amplified, the captured fluorescence image should be a perfect circle. P(C/X) provides a threshold of 0.5 to identify a circle and values above 0.5 indicate the test sample is positive. Experimental results show that P(C/X) is more effective than Δ F for determining DNA detection results. The information entropy analysis method is applied to ten mobile phones of three different brands equipped with camera sensors, which have pixel numbers ranging from 120. M to 800. M. The clinical evaluation study (n= 60) for screening hepatitis B virus (HBV) plasmid samples shows that the accuracy rate of all models of mobile phones ranges from 85% to 100%. This illustrates that successful DNA detection can be achieved using the most widely deployed electronic device. ? 2011 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983720089&doi=10.1016%2fj.bios.2011.04.036&partnerID=40&md5=7947f8dc59bb87800d815e7633fffa53 https://scholars.lib.ntu.edu.tw/handle/123456789/568538 |
ISSN: | 0956-5663 | DOI: | 10.1016/j.bios.2011.04.036 | SDG/關鍵字: | Cameras; Fluorescence; Mobile devices; Mobile phones; Mobile telecommunication systems; Polymerase chain reaction; Telephone; Telephone sets; Viruses; Accuracy rate; Camera sensor; Clinical evaluation; Commercial mobile phones; CPCR; DNA amplification; DNA detection; DNA-template; Electronic device; F values; Fluorescence image; Hepatitis B virus; Increment method; Information entropy; Information entropy method; Test samples; Threshold levels; DNA; accuracy; article; controlled study; DNA determination; DNA template; entropy; feasibility study; fluorescence analysis; gene amplification; Hepatitis B virus; image processing; mobile phone; nonhuman; plasmid; polymerase chain reaction; reliability; Hepatitis B virus |
顯示於: | 臨床醫學研究所 |
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