An immunocompetent mouse model for the tolerance of human chronic hepatitis B virus infection
Journal
Proceedings of the National Academy of Sciences of the United States of America
Journal Volume
103
Journal Issue
47
Pages
17862-17867
Date Issued
2006
Author(s)
Abstract
An animal model for human hepatitis B virus (HBV) tolerance is needed to investigate the mechanisms. This model will also facilitate therapeutic strategies for the existing 350 million patients with chronic hepatitis B. We established a mouse model by hydrodynamic injection of an engineered, replication-competent HBV DNA into the tail veins of C57BL/6 mice. In 40% of the injected mice, HBV surface antigenemia persisted for >6 months. Viral replication intermediates, transcripts, and proteins were detected in the liver tissues of the injected mice for up to 1 year. The tolerance toward HBV surface antigen in this model was shown to be due to an insufficient cellular immunity against hepatitis B core antigen, as was documented in humans. This animal model will accelerate further genetic and mechanistic studies of human chronic hepatitis B infection. ? 2006 by The National Academy of Sciences of the USA.
Subjects
DNA; HBV persistence; Hydrodynamic injection; Surface antigen tolerance
SDGs
Other Subjects
hepatitis B surface antigen; virus DNA; adoptive transfer; animal cell; animal experiment; animal model; animal tissue; article; cellular immunity; chronic hepatitis; controlled study; hepatitis B; immune response; immunological tolerance; male; mouse; nonhuman; priority journal; virus replication; Animalia; Hepatitis B virus
Publisher
National Academy of Sciences
Type
journal article