https://scholars.lib.ntu.edu.tw/handle/123456789/568697
標題: | Molecular genetic evidence supporting a novel human hepatocellular carcinoma tumor suppressor locus at 13q12.11 | 作者: | Chen C.-F. Shiou-Hwei Yeh DING-SHINN CHEN PEI-JER CHEN Jou Y.-S. |
公開日期: | 2005 | 出版社: | Wiley-Liss Inc. | 卷: | 44 | 期: | 3 | 起(迄)頁: | 320-328 | 來源出版物: | Genes Chromosomes and Cancer | 摘要: | A novel 1-cM (1.8 Mb) homozygous deletion (HD) on 13q12.11 was identified in a human hepatocellular carcinoma (HCC) cell line, SK-Hep-1, after high-density genetic marker scan and Southern blotting analysis. A loss of heterozygosity (LOH) analysis indicated that LOH frequency of the HD region in 48 pairs of HCC tissues was 52%. Interestingly, the occurrence of LOH in the 13q12.11 HD region is significantly associated with early-onset HCC, inferred from Fisher's exact test (P = 0.0047) and Mann-Whitney test (P = 0.023). Since the novel 1-cM (1.8 Mb) HD region is gene-rich with more than 37 predicted transcripts, we used a candidate gene approach by examining down-regulation of known tumor suppressor genes (TSGs), including LATS2, TG737, CRYL1, and GJB2, in HCC tissues. We detected only 14% down-regulation of the LAST2 gene that flanks the outside of the HD, in HCC tissues, by quantitative RT-PCR assays. However, we observed significant down-regulation of the TG737, CRYL1, and GJB2 genes located within the HD in 59, 64, and 71% of HCC tissues, respectively. Together, our results indicated that the identified 13q12.11 HD region contained at least three significant down-regulated TSGs, and preferential LOH in early-onset HCC patients is a putative tumor suppressor locus in HCC. ? 2005 Wiley-Liss, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984578438&doi=10.1002%2fgcc.20247&partnerID=40&md5=c9d728bbfa26cf49cb5eb1b29111dc74 https://scholars.lib.ntu.edu.tw/handle/123456789/568697 |
ISSN: | 1045-2257 | DOI: | 10.1002/gcc.20247 | SDG/關鍵字: | adult; aged; article; cancer cell culture; chromosome 13q; chromosome deletion; CRYL1 gene; down regulation; female; gene locus; genetic marker; GJB2 gene; heterozygosity loss; homozygosity; human; human cell; human tissue; LATS2 gene; liver cell carcinoma; male; molecular genetics; priority journal; quantitative analysis; rank sum test; reverse transcription polymerase chain reaction; Southern blotting; TG737 gene; tumor suppressor gene |
顯示於: | 臨床醫學研究所 |
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