https://scholars.lib.ntu.edu.tw/handle/123456789/568740
Title: | Vaccination with a DNA Vaccine Encoding Herpes Simplex Virus Type 1 VP22 Linked to Antigen Generates Long-Term Antigen-Specific CD8-Positive Memory T Cells and Protective Immunity | Authors: | Kim T.W. Hung C.-F. Kim J.W. Juang J. PEI-JER CHEN He L. Boyd D.A.K. Wu T.-C. |
Issue Date: | 2004 | Publisher: | Mary Ann Liebert Inc. | Journal Volume: | 15 | Journal Issue: | 2 | Start page/Pages: | 167-177 | Source: | Human Gene Therapy | Abstract: | Intradermal vaccination with DNA encoding herpes simplex virus type 1 (HSV-1) VP22 linked to antigen leads to spread of antigen within the epithelium and results in enhanced antigen-specific CD8+ T cell immune responses in vaccinated mice. In this study, we characterized the number of antigen-expressing dendritic cells (DCs) in the draining lymph nodes of vaccinated mice and determined whether the linkage of VP22 to antigen would influence the ability of antigen-expressing DCs to activate antigen-specific CD8+ T cells in vivo. Vaccination with DNA encoding HSV-1 VP22 linked to human papillomavirus type 16 E7 antigen generated more antigen-expressing DCs in the draining lymph nodes of vaccinated mice than E7 alone. In addition, the linkage of VP22 to E7 improved the MHC class I presentation of E7 in transfected DCs and led to enhanced activation of E7-specific CD8+ T cells. We also observed that vaccination with DNA encoding VP22 linked to E7 generated more E7-specific CD8+ memory T cells, and enhanced long-term protective antitumor immunity against an E7-expressing tumor in vaccinated mice compared with vaccination with DNA encoding E7 alone. Thus, administration of DNA encoding VP22 linked to antigen represents a plausible approach for the development of protective DNA vaccines. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984550385&doi=10.1089%2f104303404772679977&partnerID=40&md5=1a8e2f1ca34473ae17fbb5ee975cfca0 https://scholars.lib.ntu.edu.tw/handle/123456789/568740 |
ISSN: | 1043-0342 | DOI: | 10.1089/104303404772679977 | SDG/Keyword: | CD8 antigen; DNA vaccine; protein E7; protein VP22; unclassified drug; virus protein; animal experiment; antigen expression; antigen specificity; antineoplastic activity; article; cell activation; controlled study; dendritic cell; female; genetic code; genetic transfection; immunity; lymph node; major histocompatibility complex; memory cell; mouse; nonhuman; T lymphocyte; vaccination; Wart virus; Animalia; Human herpesvirus 1; Human papillomavirus; Human papillomavirus type 16; Human papillomavirus types; Papillomavirus; Simplexvirus [SDGs]SDG3 |
Appears in Collections: | 臨床醫學研究所 |
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