https://scholars.lib.ntu.edu.tw/handle/123456789/568785
標題: | Hepatitis B genotypes and precore/basal core promoter mutants in HBeAg-negative chronic hepatitis B | 作者: | Lin C.-L. Liao L.-Y. CHUN-JEN LIU PEI-JER CHEN Lai M.-Y. JIA-HORNG KAO DING-SHINN CHEN |
公開日期: | 2002 | 出版社: | Springer Japan | 卷: | 37 | 期: | 4 | 起(迄)頁: | 283-287 | 來源出版物: | Journal of Gastroenterology | 摘要: | Background. Mutations in the precore stop codon (G1896A) and the basal core promoter (A1762T and G1764A) are frequently found in hepatitis B envelope antigen (HBeAg)-negative chronic hepatitis B. However, the clinical significance of these mutations remains controversial. We therefore investigated the influence of hepatitis B virus (HBV) genotypes, as well as precore/basal core promoter mutants, on the clinical and virological features of patients with HBeAg-negative chronic hepatitis B. Methods. Serum samples from 37 patients with HBeAg-negative chronic hepatitis B were collected for serological and molecular assays. The precore and basal core promoter regions were amplified by polymerase chain reaction and the amplicons were directly sequenced and analyzed. HBV genotype was determined by polymerase chain reaction-restriction fragment length polymorphism. Results. Most of the patients had detectable serum HBV DNA, and genotypes B and C were the predominant strains. The overall prevalence of the precore stop codon mutant and basal core promoter mutant was 67% and 60%, respectively. The baseline clinical and virological features of patients with genotype B and genotype C infection were comparable. However, in the patients with precore/basal core promoter dual mutations there was a significantly lower proportion of individuals with a high detectable serum HBV DNA level (>100 pg/ml) than in the patients with either the precore stop codon mutation alone or the basal core promoter mutation alone (P = 0.04 by the logistic regression test for the trend). Conclusions. Our data suggest a high prevalence of precore stop codon and basal core promoter mutation in Taiwanese patients with HBeAg-negative chronic hepatitis B, and the influence of the basal core promoter mutation on HBV replication is modulated by the emergence of the precore stop codon mutation. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984533424&doi=10.1007%2fs005350200036&partnerID=40&md5=9f45b813b2aebd30fea4578153b0b094 https://scholars.lib.ntu.edu.tw/handle/123456789/568785 |
ISSN: | 0944-1174 | DOI: | 10.1007/s005350200036 | SDG/關鍵字: | hepatitis B(e) antigen; virus DNA; adult; aged; amplicon; article; blood level; chronic hepatitis; clinical article; clinical feature; controlled study; DNA determination; female; gene amplification; gene mutation; gene sequence; genetic analysis; genotype; hepatitis B; Hepatitis B virus; human; logistic regression analysis; male; polymerase chain reaction; prevalence; priority journal; promoter region; restriction fragment length polymorphism; serology; stop codon; Taiwan; virus replication; virus strain |
顯示於: | 臨床醫學研究所 |
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