https://scholars.lib.ntu.edu.tw/handle/123456789/568787
標題: | Naturally occurring hepatitis B surface gene variants in chronic hepatitis B virus infection: Correlation with viral serotypes and clinical stages of liver disease | 作者: | CHUN-JEN LIU JIA-HORNG KAO Shau W.-Y. PEI-JER CHEN Lai M.-Y. DING-SHINN CHEN |
公開日期: | 2002 | 出版社: | Wiley-Liss Inc. | 卷: | 68 | 期: | 1 | 起(迄)頁: | 50-59 | 來源出版物: | Journal of Medical Virology | 摘要: | Virus variants escaping from host immunity may be implicated in the pathogenesis of hepatitis B virus (HBV) infection. In this cross-sectional study, the association was evaluated of the frequency of amino acid variation within the immunogenic epitopes of surface gene with different disease stages of chronic HBV infection. The surface gene of HBV encompassing the a determinant (amino acids 124-148) and the putative HLA class I restricted cytotoxic T lymphocyte (CTL) epitope (amino acids 28-51) were amplified and directly sequenced in 33 asymptomatic carriers (Group I), 31 patients with chronic hepatitis (Group II), 22 with cirrhosis (Group III), and 36 with hepatocellular carcinoma (Group IV). The amino acid sequences were compared subsequently with the consensus sequences of HBV serotype adw or adr. The frequency of amino acid variation per site per sequence (FEQ) was analyzed by generalized estimating equation with Poisson model after stratification by clinical and virological features. The FEQ was 1.21% overall, and was highest in Group IV patients and in patients above 50 years of age. In contrast, nine Group IV patients aged below 50 years who were infected with serotype adw had an inversely higher FEQ than those above 50; the age effect among hepatocellular carcinoma patients was significantly different from that among non-cancerous patients (P = 0.04). Variation of amino acid clustered within a determinant and CTL epitope for serotype adw but was distributed at random for serotype adr. Mutation hotspots differed between serotypes adw and adr. The FEQ of HBV surface protein is correlated positively with advancing age and severity of liver disease, and certain variants may contribute to the persistence of HBV infection. ? 2002 Wiley-Liss, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036021198&doi=10.1002%2fjmv.10169&partnerID=40&md5=dc70a02b692559007b56dda5129209a4 https://scholars.lib.ntu.edu.tw/handle/123456789/568787 |
ISSN: | 0146-6615 | DOI: | 10.1002/jmv.10169 | SDG/關鍵字: | epitope; virus DNA; epitope; hepatitis B surface antigen; adult; amino acid sequence; article; chronic hepatitis; cytotoxic T lymphocyte; hepatitis B; Hepatitis B virus; human; liver cell carcinoma; liver cirrhosis; major clinical study; nonhuman; nucleotide sequence; polymerase chain reaction; cross-sectional study; female; genetic variability; genetics; hepatitis B; liver cell carcinoma; liver cirrhosis; liver tumor; male; middle aged; molecular genetics; nucleotide sequence; pathophysiology; serotyping; virology; virus gene; Hepatitis B virus; Adult; Amino Acid Sequence; Base Sequence; Carcinoma, Hepatocellular; Cross-Sectional Studies; DNA, Viral; Epitopes, B-Lymphocyte; Epitopes, T-Lymphocyte; Female; Genes, Viral; Hepatitis B Surface Antigens; Hepatitis B virus; Hepatitis B, Chronic; Humans; Liver Cirrhosis; Liver Neoplasms; Male; Middle Aged; Molecular Sequence Data; Serotyping; Variation (Genetics) |
顯示於: | 臨床醫學研究所 |
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