https://scholars.lib.ntu.edu.tw/handle/123456789/568811
標題: | Liver and peripheral blood mononuclear cells are not major sites for GB virus-C/hepatitis G virus replication | 作者: | JIA-HORNG KAO Chen W. PEI-JER CHEN Lai M.-Y. DING-SHINN CHEN |
公開日期: | 1999 | 卷: | 144 | 期: | 11 | 起(迄)頁: | 2173-2183 | 來源出版物: | Archives of Virology | 摘要: | The pathogenesis and replication sites of GB virus-C/hepatitis G virus (GBV-C/HGV) in humans remain unclear. The presence of GBV-C/HGV and hepatitis C virus (HCV) RNA sequences in matched serum, peripheral blood mononuclear cells (PBMC) and liver samples in 10 patients with GBV-C/HGV infection, 8 of whom were coinfected with HCV was explored. Positive- and negative-strand GBV-C/HGV and HCV RNA were detected by strand-specific reverse-transcription polymerase chain reaction (RT-PCR), and virus titers were quantified by competitive PCRs. Positive-strand GBV-C/HGV RNA was detected in 7 of 10 PBMC samples of the patients with serum GBV-C/HGV RNA, but negative-strand GBV-C/HGV RNA was not found in these cells. Positive-strand GBV-C/HGV RNA was found in 9 liver samples, and 2 (22%) of them also had negative strand. In contrast, negative-strand HCV RNA was frequently found in PBMC and liver samples. A positive correlation between the titer of viral RNA in liver tissue and that in serum sample was demonstrated in HCV infection, but not in GBV-C/HGV infection. These findings suggest that liver and PBMC are not the major replication sites for GBV-C/HGV and that GBV-C/HGV is not a primary hepatotropic virus. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0032721245&doi=10.1007%2fs007050050631&partnerID=40&md5=5c99d585424cb246e0c832f41b7ad6c9 https://scholars.lib.ntu.edu.tw/handle/123456789/568811 |
ISSN: | 0304-8608 | DOI: | 10.1007/s007050050631 | SDG/關鍵字: | abaca; blood cell; hepatitis G virus; hepatitis gb virus c; reverse transcription polymerase chain reaction; virus infection; virus replication; Adult; Aged; Female; Flaviviridae; Hepatitis C; Hepatitis, Viral, Human; Humans; Leukocytes, Mononuclear; Liver; Male; Middle Aged; RNA, Viral; Virus Replication; GB virus C/Hepatitis G virus; Hepatitis C virus; Musa textilis; RNA viruses |
顯示於: | 臨床醫學研究所 |
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