https://scholars.lib.ntu.edu.tw/handle/123456789/568818
標題: | Comparison of two methods for quantification of hepatitis B viral DNA | 作者: | JIA-HORNG KAO Wood M. PEI-JER CHEN Lai M.-Y. DING-SHINN CHEN |
公開日期: | 1999 | 出版社: | Blackwell Publishing | 卷: | 14 | 期: | 5 | 起(迄)頁: | 423-426 | 來源出版物: | Journal of Gastroenterology and Hepatology (Australia) | 摘要: | Background: Quantitation of serum hepatitis B virus (HBV) DNA has proven useful in assisting with patient management and treatment and several commercially available assays have been developed to quantify serum HBV-DNA levels. Methods: The performance of the Cross-linking assay and the branched- DNA signal amplification (bDNA) assay for the quantitative measurement of HBV-DNA was studied in 99 hepatitis B surface antisen-positive and viraemic patients. Results: Of these samples, 82 (83%) were positive for HBV-DNA by both assays and four (4%) were below the cut-off for both assays. Of the remaining 13 samples, 10 contained measurable levels of HBV-DNA by the cross- linking assay alone and three other samples contained measurable levels of HBV-DNA by the bDNA assay alone. The sensitivity gain of the cross-linking assay relative to bDNA assay in this study population was 10/92 (11%). In addition, a linear regression analysis showed that the HBV-DNA levels obtained from both assays was significantly correlated (γ = 0.974, P < 0.0001). Conclusions: These findings suggest that the recently developed cross-linking assay is more sensitive than the bDNA assay for the quantitative determination of HBV-DNA. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984539045&doi=10.1046%2fj.1440-1746.1999.01885.x&partnerID=40&md5=d825602d7c0c411d433f7571e597a788 https://scholars.lib.ntu.edu.tw/handle/123456789/568818 |
ISSN: | 0815-9319 | DOI: | 10.1046/j.1440-1746.1999.01885.x | SDG/關鍵字: | virus DNA; article; controlled study; DNA cross linking; DNA determination; hepatitis B; Hepatitis B virus; human; intermethod comparison; major clinical study; priority journal; quantitative diagnosis; viremia; virus titration |
顯示於: | 臨床醫學研究所 |
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