https://scholars.lib.ntu.edu.tw/handle/123456789/568841
標題: | Inhibition of hepatitis delta virus genomic ribozyme self-cleavage by aminoglycosides | 作者: | Chia J.-S. Wu H.-L. Wang H.-W. Chen D.-S. PEI-JER CHEN |
關鍵字: | Aminoglycosides; Chemical modification; HDV; Inhibition; Ribozyme | 公開日期: | 1997 | 出版社: | BioMed Central Ltd. | 卷: | 4 | 期: | 5 | 起(迄)頁: | 208-216 | 來源出版物: | Journal of Biomedical Science | 摘要: | Subgenomic regions of hepatitis delta virus (HDV) RNA contains ribozyme whose activities are important to viral life cycles and depend on a unique pseudoknot structure. To explore the characters of HDV ribozyme, antibiotics of the aminoglycoside, which has been shown inhibiting self-splicing of group I intron and useful in elucidating its structure, were tested for their effect on HDV genomic ribozyme. Aminoglycosides, including tobramycin, netromycin, neomycin and gentamicin effectively inhibited HDV genomic ribozyme self-cleavage in vitro at a concentration comparable to that inhibiting group I intron self-splicing. The extent of inhibition depended upon the concentration of magnesium ion. Chemical modification mapping of HDV ribozyme RNA indicated that the susceptibility of nucleotide 703 to the modifying agent was enhanced in the presence of tobramycin, suggesting a conformational shift of HDV ribozyme, probably due to an interaction with the aminoglycoside. Finally, we examined the effect of aminoglycoside on HDV cleavage and replication in cell lines, however, none of the aminoglycoside effective in vitro exerted suppressive effects in vivo. Our results represented as an initial effort in utilizing aminoglycoside to probe the structure of HDV ribozyme and to compare its reaction mechanism with those of other related ribozymes. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984575713&doi=10.1007%2fBF02253420&partnerID=40&md5=32bfe8be0f2741fc6d9691045cb21748 https://scholars.lib.ntu.edu.tw/handle/123456789/568841 |
ISSN: | 1021-7770 | DOI: | 10.1007/BF02253420 | SDG/關鍵字: | aminoglycoside; ribozyme; antiviral activity; article; cell division; Hepatitis delta virus; intron; nonhuman; priority journal; RNA structure; virus inhibition |
顯示於: | 臨床醫學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。