https://scholars.lib.ntu.edu.tw/handle/123456789/568841
Title: | Inhibition of hepatitis delta virus genomic ribozyme self-cleavage by aminoglycosides | Authors: | Chia J.-S. Wu H.-L. Wang H.-W. Chen D.-S. PEI-JER CHEN |
Keywords: | Aminoglycosides; Chemical modification; HDV; Inhibition; Ribozyme | Issue Date: | 1997 | Publisher: | BioMed Central Ltd. | Journal Volume: | 4 | Journal Issue: | 5 | Start page/Pages: | 208-216 | Source: | Journal of Biomedical Science | Abstract: | Subgenomic regions of hepatitis delta virus (HDV) RNA contains ribozyme whose activities are important to viral life cycles and depend on a unique pseudoknot structure. To explore the characters of HDV ribozyme, antibiotics of the aminoglycoside, which has been shown inhibiting self-splicing of group I intron and useful in elucidating its structure, were tested for their effect on HDV genomic ribozyme. Aminoglycosides, including tobramycin, netromycin, neomycin and gentamicin effectively inhibited HDV genomic ribozyme self-cleavage in vitro at a concentration comparable to that inhibiting group I intron self-splicing. The extent of inhibition depended upon the concentration of magnesium ion. Chemical modification mapping of HDV ribozyme RNA indicated that the susceptibility of nucleotide 703 to the modifying agent was enhanced in the presence of tobramycin, suggesting a conformational shift of HDV ribozyme, probably due to an interaction with the aminoglycoside. Finally, we examined the effect of aminoglycoside on HDV cleavage and replication in cell lines, however, none of the aminoglycoside effective in vitro exerted suppressive effects in vivo. Our results represented as an initial effort in utilizing aminoglycoside to probe the structure of HDV ribozyme and to compare its reaction mechanism with those of other related ribozymes. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984575713&doi=10.1007%2fBF02253420&partnerID=40&md5=32bfe8be0f2741fc6d9691045cb21748 https://scholars.lib.ntu.edu.tw/handle/123456789/568841 |
ISSN: | 1021-7770 | DOI: | 10.1007/BF02253420 | SDG/Keyword: | aminoglycoside; ribozyme; antiviral activity; article; cell division; Hepatitis delta virus; intron; nonhuman; priority journal; RNA structure; virus inhibition |
Appears in Collections: | 臨床醫學研究所 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.