https://scholars.lib.ntu.edu.tw/handle/123456789/569131
Title: | Activation of transforming growth factor-β1 by thrombin via integrins αvβ1, αvβ3, and αvβ5 in buccal fibroblasts: Suppression by epigallocatechin-3-gallate | Authors: | JENNY ZWEI-CHIENG CHANG Hsieh Y.-P. Lin W.-H. HSIN-MING CHEN YEN-PING KUO |
Issue Date: | 2017 | Publisher: | John Wiley and Sons Inc. | Journal Volume: | 39 | Journal Issue: | 7 | Start page/Pages: | 1436-1445 | Source: | Head and Neck | Abstract: | Background: Transforming growth factor-beta (TGF-β) plays a central role in the pathogenesis of oral submucous fibrosis (OSF). Thrombin is a key player in tissue repair, inflammation, and fibrosis after injury. Methods: Effects of thrombin on activated-TGF-β1 levels, Smad3 phosphorylation, and connective tissue growth factor (CTGF/CCN2) synthesis in primary human buccal mucosal fibroblasts (BMFs) were assessed by enzyme-linked immunosorbent assay or Western blot analysis. Results: Thrombin and protease-activated receptor-1 (PAR-1) agonist induced TGF-β1 activation and Smad3 phosphorylation. Pretreatment with TGF-β-neutralizing antibody completely inhibited thrombin-induced CCN2 synthesis. Neutralizing antibodies to integrin αv, β1, αvβ3, αvβ5, and Rho-associated coiled-coil forming protein kinase (ROCK) inhibitor Y27632 completely blocked thrombin-induced TGF-β1 activation, Smad3 phosphorylation, and CCN2 synthesis. Epigallocatechin-3-gallate (EGCG) dose-dependently inhibited thrombin-induced TGF-β1 activation. Conclusion: Thrombin induces αvβ1, αvβ3, and αvβ5 integrins-mediated TGF-β1 activations via ROCK signaling. EGCG inhibits thrombin-induced CCN2 synthesis in BMFs by suppressing latent TGF-β1 activation. ? 2017 Wiley Periodicals, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019000309&doi=10.1002%2fhed.24791&partnerID=40&md5=d36068a9ede0c5ecfb56f5a711e5f94b https://scholars.lib.ntu.edu.tw/handle/123456789/569131 |
ISSN: | 1043-3074 | DOI: | 10.1002/hed.24791 | SDG/Keyword: | 4 (1 aminoethyl) n (4 pyridyl)cyclohexanecarboxamide; alphaVbeta1 integrin; alphaVbeta5 integrin; connective tissue growth factor; epigallocatechin gallate; integrin; neutralizing antibody; Smad3 protein; thrombin; transforming growth factor beta1; unclassified drug; vitronectin receptor; alphaVbeta5 integrin; catechin; epigallocatechin gallate; integrin alphavbeta1; thrombin; transforming growth factor beta1; vitronectin receptor; Article; cheek cell; controlled study; enzyme linked immunosorbent assay; fibroblast; human; human cell; priority journal; protein phosphorylation; protein synthesis; Western blotting; analogs and derivatives; cell culture; drug effects; fibroblast; metabolism; mouth disease; mouth mucosa; pathology; procedures; sensitivity and specificity; Blotting, Western; Catechin; Cells, Cultured; Enzyme-Linked Immunosorbent Assay; Fibroblasts; Humans; Integrin alphaVbeta3; Mouth Mucosa; Oral Submucous Fibrosis; Receptors, Vitronectin; Sensitivity and Specificity; Thrombin; Transforming Growth Factor beta1 [SDGs]SDG3 |
Appears in Collections: | 牙醫學系 |
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