https://scholars.lib.ntu.edu.tw/handle/123456789/569177
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Yeh C.-C. | en_US |
dc.contributor.author | Hsieh H.-L. | en_US |
dc.contributor.author | Lee J. | en_US |
dc.contributor.author | Jan Y.-H. | en_US |
dc.contributor.author | Lai T.-C. | en_US |
dc.contributor.author | Hong C.-Y. | en_US |
dc.contributor.author | Hsiao M. | en_US |
dc.contributor.author | YEN-PING KUO | en_US |
dc.date.accessioned | 2021-07-05T05:42:09Z | - |
dc.date.available | 2021-07-05T05:42:09Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1043-3074 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79955927660&doi=10.1002%2fhed.21555&partnerID=40&md5=325f0e3922c34e8ab4c1bc56d5833956 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/569177 | - |
dc.description.abstract | Background. PUMA (a p53 up-regulated modulator of apoptosis) is induced by p53 tumor suppressor and other apoptotic stimuli. It was found to be a principal mediator of cell death in response to diverse apoptotic signals, implicating PUMA as a likely tumor suppressor. Methods. In this study, we examined the efficacy of targeted PUMA gene therapy in human oral cancer (SAS) cells using polyethylenimine (PEI)-mediated transfection for gene delivery. Results. Exogenous expression of PUMA in SAS cells resulted in apoptosis with cytochrome c release, activation of caspase-3 and -9, and cleavage of PARP. Gene delivery of PEI/PUMA in SAS xenografts induced apoptosis and resulted in significant reductions (?60%) of tumor growth in vivo. Furthermore, we have shown that PEI-mediated PUMA gene therapy prolonged survival of animals with orthotopic SAS oral cancers. Conclusions. Taken together, these results indicated that PUMA gene therapy via PEI delivery could be a promising method for the treatment of oral squamous cell carcinoma. Copyright ? 2010 Wiley Periodicals, Inc. | - |
dc.relation.ispartof | Head and Neck | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | caspase 3; caspase 9; cytochrome c; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; polyethyleneimine; PUMA protein; animal experiment; animal model; animal tissue; apoptosis; article; cancer cell; cancer inhibition; cancer survival; enzyme activation; enzyme release; gene expression; gene therapy; genetic transfection; human; human cell; mouse; mouth cancer; nonhuman; nonviral gene delivery system; priority journal; tumor growth; Analysis of Variance; Animals; Apoptosis; Apoptosis Regulatory Proteins; Carcinoma, Squamous Cell; Caspase 3; Cytochromes c; Disease Models, Animal; Gene Therapy; Genetic Vectors; Humans; In Situ Nick-End Labeling; Kaplan-Meier Estimate; Mice; Mice, SCID; Mouth Neoplasms; Neoplasms, Experimental; Polyethyleneimine; Random Allocation; Sensitivity and Specificity; Statistics, Nonparametric; Survival Rate; Transfection; Transplantation, Heterologous; Tumor Cells, Cultured; Tumor Suppressor Proteins | - |
dc.title | Polyethylenimine-mediated PUMA gene delivery to orthotopic oral cancer: Suppression of tumor growth through apoptosis induction in situ and prolonged survival | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1002/hed.21555 | - |
dc.identifier.pmid | 20737492 | - |
dc.identifier.scopus | 2-s2.0-79955927660 | - |
dc.relation.pages | 878-885 | - |
dc.relation.journalvolume | 33 | - |
dc.relation.journalissue | 6 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Dentistry | - |
crisitem.author.dept | School of Dentistry | - |
crisitem.author.dept | Dentistry-NTUH | - |
crisitem.author.orcid | 0000-0001-8321-2575 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 牙醫學系 |
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