https://scholars.lib.ntu.edu.tw/handle/123456789/569177
Title: | Polyethylenimine-mediated PUMA gene delivery to orthotopic oral cancer: Suppression of tumor growth through apoptosis induction in situ and prolonged survival | Authors: | Yeh C.-C. Hsieh H.-L. Lee J. Jan Y.-H. Lai T.-C. Hong C.-Y. Hsiao M. YEN-PING KUO |
Issue Date: | 2011 | Journal Volume: | 33 | Journal Issue: | 6 | Start page/Pages: | 878-885 | Source: | Head and Neck | Abstract: | Background. PUMA (a p53 up-regulated modulator of apoptosis) is induced by p53 tumor suppressor and other apoptotic stimuli. It was found to be a principal mediator of cell death in response to diverse apoptotic signals, implicating PUMA as a likely tumor suppressor. Methods. In this study, we examined the efficacy of targeted PUMA gene therapy in human oral cancer (SAS) cells using polyethylenimine (PEI)-mediated transfection for gene delivery. Results. Exogenous expression of PUMA in SAS cells resulted in apoptosis with cytochrome c release, activation of caspase-3 and -9, and cleavage of PARP. Gene delivery of PEI/PUMA in SAS xenografts induced apoptosis and resulted in significant reductions (?60%) of tumor growth in vivo. Furthermore, we have shown that PEI-mediated PUMA gene therapy prolonged survival of animals with orthotopic SAS oral cancers. Conclusions. Taken together, these results indicated that PUMA gene therapy via PEI delivery could be a promising method for the treatment of oral squamous cell carcinoma. Copyright ? 2010 Wiley Periodicals, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-79955927660&doi=10.1002%2fhed.21555&partnerID=40&md5=325f0e3922c34e8ab4c1bc56d5833956 https://scholars.lib.ntu.edu.tw/handle/123456789/569177 |
ISSN: | 1043-3074 | DOI: | 10.1002/hed.21555 | SDG/Keyword: | caspase 3; caspase 9; cytochrome c; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase; polyethyleneimine; PUMA protein; animal experiment; animal model; animal tissue; apoptosis; article; cancer cell; cancer inhibition; cancer survival; enzyme activation; enzyme release; gene expression; gene therapy; genetic transfection; human; human cell; mouse; mouth cancer; nonhuman; nonviral gene delivery system; priority journal; tumor growth; Analysis of Variance; Animals; Apoptosis; Apoptosis Regulatory Proteins; Carcinoma, Squamous Cell; Caspase 3; Cytochromes c; Disease Models, Animal; Gene Therapy; Genetic Vectors; Humans; In Situ Nick-End Labeling; Kaplan-Meier Estimate; Mice; Mice, SCID; Mouth Neoplasms; Neoplasms, Experimental; Polyethyleneimine; Random Allocation; Sensitivity and Specificity; Statistics, Nonparametric; Survival Rate; Transfection; Transplantation, Heterologous; Tumor Cells, Cultured; Tumor Suppressor Proteins |
Appears in Collections: | 牙醫學系 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.