Esculetin enhances TRAIL-induced apoptosis through DR5 upregulation in human oral cancer SAS cells
Journal
Oral Oncology
Journal Volume
45
Journal Issue
12
Pages
1067-1072
Date Issued
2009
Author(s)
Abstract
Esculetin has been shown to selectively induce tumor apoptosis in several types of cancers and is regarded as a promising chemotherapeutic agent. In this study, we showed that esculetin significantly suppressed the growth of oral cancer SAS cells in a dose-dependent manner. DNA content flow cytometry and TUNEL assay revealed that esculetin induced cell cycle arrest and apoptosis. Western blotting showed esculetin increased DR5 protein expression and activated caspase-8, which differed from previous studies conducted in other cell types. Furthermore, treatment with esculetin significantly increased TRAIL-induced apoptosis in SAS cells and the TRAIL-sensitizing effect was blocked by DR5/Fc chimera protein. Our results indicate that esculetin enhances TRAIL-induced apoptosis primarily through upregulation of DR5. Combination of esculetin and TRAIL may be a novel treatment strategy for oral cancers. ? 2009 Elsevier Ltd. All rights reserved.
SDGs
Other Subjects
caspase 8; death receptor 5; DNA; esculetin; tumor necrosis factor related apoptosis inducing ligand; apoptosis; article; cancer cell; cancer growth; cell cycle arrest; cell growth; cell type; controlled study; dose response; enzyme activation; flow cytometry; human; human cell; mouth cancer; nick end labeling; priority journal; protein expression; Western blotting; Antioxidants; Apoptosis; Blotting, Western; Caspase 8; Cell Cycle; Flow Cytometry; Humans; In Situ Nick-End Labeling; Mouth Neoplasms; Neoplasm Proteins; Receptors, TNF-Related Apoptosis-Inducing Ligand; Umbelliferones
Type
journal article