|Title:||Mutations of the adenomatous polyposis coli gene in areca quid and tobacco-associated oral squamous cell carcinomas in Taiwan||Authors:||SANG-HENG KOK
|Issue Date:||2002||Journal Volume:||31||Journal Issue:||7||Start page/Pages:||395-401||Source:||Journal of Oral Pathology and Medicine||Abstract:||
Background: Adenomatous polyposis coli (APC) gene mutations have been demonstrated not only in colorectal tumors but also in a variety of human cancers. Methods: To elucidate the possible roles of APC gene mutations in oral squamous cell carcinomas (OSCCs), we examined 40 untreated human primary OSCCs using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing assays. Results: By screening nearly one-half of the coding region (codons 279-1673, including the MCR) of the APC gene, five missense mutations and a 1-base pair deletion were detected in five (12.5%) tumors, resulting in five amino-acid substitutions or a truncation of the APC protein. All patients with APC mutations were both areca quid chewers and tobacco smokers (P = 0.049). Conclusions: These results suggest that APC mutations may also contribute to the carcinogenesis of at least some OSCCs in Taiwan, especially for the users of areca quid and tobacco.
|ISSN:||0904-2512||DOI:||10.1034/j.1600-0714.2002.00118.x||SDG/Keyword:||Adenomatous Polyposis Coli Protein; Adult; Aged; Aged, 80 and over; Amino Acid Substitution; Areca; Base Pairing; Carcinoma, Squamous Cell; Chi-Square Distribution; Codon; Exons; Female; Frameshift Mutation; Gene Deletion; Genes, APC; Humans; Loss of Heterozygosity; Male; Middle Aged; Mouth Neoplasms; Mutation; Mutation, Missense; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Sequence Analysis, DNA; Smoking; Statistics; Taiwan
|Appears in Collections:||牙醫學系|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.