https://scholars.lib.ntu.edu.tw/handle/123456789/569243
標題: | Eugenol Triggers Different Pathobiological Effects on Human Oral Mucosal Fibroblasts 1 | 作者: | JIIANG-HUEI JENG Hahn, Liangjiunn Lu, F. J. Wang, Yingjang YEN-PING KUO |
公開日期: | 1994 | 卷: | 73 | 期: | 5 | 起(迄)頁: | 1050-1055 | 來源出版物: | Journal of Dental Research | 摘要: | Pathobiological effects of eugenol (4-allyl-2-methoxyphenol), a major constituent of betel quid (BQ), were studied on oral mucosal fibroblasts. At a concentration higher than 3 mmol/L, eugenol was cytotoxic to oral mucosal fibroblasts in a concentration-and time-dependent manner. Cell death was associated with intracellular depletion of glutathione (GSH). Most of the GSH was depleted prior to the onset of cell death. At concentrations of 3 mmol/L and 4 mmol/L, eugenol depleted about 45% and 77% of GSH after one-hour incubation. In addition, eugenol decreased cellular ATP level in a concentration- and time-dependent manner. Eugenol also inhibited lipid peroxidation. Inhibition of lipid peroxidation was partially explained by its dose-dependent inhibition of xanthine oxidase activity. The IC50 of eugenol on xanthine oxidase activity was about 0.3 mmol/L. No DNA strand break activity for eugenol was found at concentrations between 0.5 and 3 mmol/L. Taken together, frequent exposure of oral mucosa to a high concentration of eugenol during the chewing of BQ might be involved in the pathogenesis of oral submucous fibrosis and oral cancer via its cytotoxicity. In contrast, eugenol at a concentration less than 1 mmol/L might protect cells from the genetic attack of reactive oxygen species via inhibition of xanthine oxidase activity and lipid peroxidation. ? 1994, SAGE Publications. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028433013&doi=10.1177%2f00220345940730050601&partnerID=40&md5=43f0462ef2d7599d8cc0eb2a4c7d2da9 https://scholars.lib.ntu.edu.tw/handle/123456789/569243 |
ISSN: | 0022-0345 | DOI: | 10.1177/00220345940730050601 | SDG/關鍵字: | adenosine triphosphate; eugenol; glutathione; mutagenic agent; xanthine oxidase; article; betel nut; chemistry; DNA damage; dose response; drug effect; fibroblast; human; lipid peroxidation; medicinal plant; mouth mucosa; mutagen testing; Adenosine Triphosphate; Areca; DNA Damage; Dose-Response Relationship, Drug; Eugenol; Fibroblasts; Glutathione; Human; Lipid Peroxidation; Mouth Mucosa; Mutagenicity Tests; Mutagens; Plants, Medicinal; Support, Non-U.S. Gov't; Xanthine Oxidase |
顯示於: | 牙醫學系 |
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