https://scholars.lib.ntu.edu.tw/handle/123456789/569473
標題: | The intracellular domain of amyloid precursor protein interacts with FKBP12 | 作者: | Liu F.-L. Liu P.-H. Shao H.-W. FAN-LU KUNG |
關鍵字: | AICD (APP intracellular domain); Alzheimer's disease; FK506; FKBPs | 公開日期: | 2006 | 卷: | 350 | 期: | 2 | 起(迄)頁: | 472-477 | 來源出版物: | Biochemical and Biophysical Research Communications | 摘要: | To elucidate the roles of the APP intracellular domain (AICD) in the development of Alzheimer's disease, a yeast two-hybrid system was used to screen for AICD-interacting proteins. Our result revealed that FKBP12, an immunophilin with a peptidyl-prolyl cis-trans isomerase (PPIase) activity, may interact with AICD. This interaction was confirmed by coimmunoprecipitation studies. FKBP12 has been shown to be expressed at a higher level in areas of pathology of patients with neurodegenerative diseases. In addition, Pin1, a member of another PPIase family, has been suggested to be involved in the amyloidogenic APP processing and Aβ production. The interaction between FKBP12 and AICD might hint at a possible role FKBP12 plays, probably in a fashion similar to Pin1, in the amyloidogenesis of APP. We also found that the interaction was interfered, in a dose-dependent manner, by FK506, whose neuroprotective effect has been suggested to be correlated with its PPIase inhibitory activity. ? 2006 Elsevier Inc. All rights reserved. |
URI: | 2-s2.0-33749324414 https://scholars.lib.ntu.edu.tw/handle/123456789/569473 |
ISSN: | 0006291X | DOI: | 10.1016/j.bbrc.2006.09.073 | SDG/關鍵字: | amyloid beta protein; amyloid precursor protein; cyclophilin; fk 506 binding protein; tacrolimus; Alzheimer disease; article; concentration response; controlled study; correlation analysis; degenerative disease; enzyme activity; enzyme inhibition; human; human cell; immunoprecipitation; neuroprotection; nucleotide sequence; pathology; priority journal; protein domain; protein expression; protein function; protein interaction; screening; Amyloid beta-Protein Precursor; Binding Sites; Humans; Immunoprecipitation; Protein Structure, Tertiary; Recombinant Fusion Proteins; Tacrolimus Binding Protein 1A; Two-Hybrid System Techniques |
顯示於: | 藥學系 |
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