https://scholars.lib.ntu.edu.tw/handle/123456789/569475
標題: | The intracellular domain of amyloid precursor protein interacts with flotillin-1, a lipid raft protein | 作者: | Chen T.-Y. Liu P.-H. Ruan C.-T. Chiu L. FAN-LU KUNG |
關鍵字: | AICD (APP intracellular domain); Alzheimer's disease; Flotillin; Lipid rafts | 公開日期: | 2006 | 卷: | 342 | 期: | 1 | 起(迄)頁: | 266-272 | 來源出版物: | Biochemical and Biophysical Research Communications | 摘要: | Amyloid β (Aβ) is a pathological hallmark of Alzheimer's disease (AD). It is derived from the amyloid precursor protein (APP) by two sequential proteolytic cleavages, which also generate the APP intracellular domain (AICD). The precise cellular function(s) of AICD still remain obscure. To elucidate the roles of AICD in the development of AD, a yeast two-hybrid system was used to screen a human brain cDNA library for proteins interacting directly with AICD. One of the potential AICD-interacting proteins identified from our screening result is a lipid raft-associated protein, flotillin-1. The interaction was confirmed by glutathione S-transferase pull-down and coimmunoprecipitation studies. Since lipid raft has been suggested to play an important role in signal transduction as well as the pathogenic development of neurodegenerative diseases, it is proposed that flotillin-1 may recruit APP to lipid rafts and therefore participate in the localization and processing of APP. ? 2006 Elsevier Inc. All rights reserved. |
URI: | 2-s2.0-32644463736 https://scholars.lib.ntu.edu.tw/handle/123456789/569475 |
ISSN: | 0006291X | DOI: | 10.1016/j.bbrc.2006.01.156 | SDG/關鍵字: | amyloid precursor protein; flotillin 1; glutathione transferase; protein derivative; unclassified drug; Alzheimer disease; article; DNA library; immunoprecipitation; lipid raft; priority journal; protein domain; protein function; protein protein interaction; screening test; signal transduction; two hybrid system; Amyloid beta-Protein Precursor; Gene Expression; Humans; Membrane Microdomains; Membrane Proteins; Protein Binding; Protein Structure, Tertiary; Saccharomyces cerevisiae; Two-Hybrid System Techniques |
顯示於: | 藥學系 |
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