Differences in oral habit and lymphocyte subpopulation affect malignant transformation of patients with oral precancer
Journal
Journal of the Formosan Medical Association
Journal Volume
115
Journal Issue
4
Pages
263-268
Date Issued
2016
Author(s)
Yeh C.-Y.
Lin C.-L.
Chang M.-C.
Kuo Y.-S.
Hahn L.-J.
Chan C.-P.
Abstract
Background/purpose: In Taiwan, the combination of betel quid chewing, alcohol consumption, and smoking habits increases oral cancer risk by 123-fold compared to persons without these habits. Lymphocyte populations in patients may potentially affect the malignant transformation of oral precancer. Methods: A total of 28 patients with oral precancer from our previous cohort were enrolled in this study, and their personal information and oral habits were documented. Their lymphocyte populations (CD4+, CD8+, CD19+, and CD56+) and activation markers (CD25 and CD69) were determined by flow cytometry from 1999 to 2004. After follow up till December 2014, data of patients with/without malignant transformation were recorded, and the relation between oral habits and percentage of initial lymphocyte markers was evaluated using the Student t test and Fisher's exact test. Results: Ten precancer patients developed oral squamous cell carcinoma with a mean period of malignant transformation of 6.8 ± 2.1 years. Patients with malignant transformation had a mean age of 48.4 ± 5.0 years (n = 10), relatively more than that of patients without malignant transformation (41.6 ± 6.3 years, n = 18) (p < 0.05). An increase was noted in the population of peripheral blood mononuclear cells expressing CD4+CD69+, CD19+CD69+, and CD56+CD69+ (p < 0.05) in precancer patients with malignant transformation. Alcohol consumption showed an association with the malignant transformation of patients with precancer (p = 0.030), whereas betel quid and smoking showed little effect. Conclusion: These results suggest that age, alcohol consumption, and early activation of T cells, B cells, and natural killer cells are crucial in the malignant transformation of oral precancer. Analysis of patient's lymphocyte populations may help predict the malignant transformation of oral precancer. ? 2015 Formosan Medical Association.
SDGs
Other Subjects
CD19 antigen; CD56 antigen; CD69 antigen; lymphocyte surface marker; biological marker; adult; alcohol consumption; Article; betel nut; CD25+ T lymphocyte; CD4+ T lymphocyte; CD8+ T lymphocyte; clinical article; controlled study; human; human cell; lymphocyte subpopulation; male; malignant transformation; mouth hygiene; mouth squamous cell carcinoma; peripheral blood mononuclear cell; precancer; protein expression; smoking; adverse effects; Areca; B lymphocyte; Carcinoma, Squamous Cell; drinking behavior; immunology; lymphocyte subpopulation; middle aged; Mouth Neoplasms; natural killer cell; Taiwan; Adult; Alcohol Drinking; Areca; B-Lymphocytes; Biomarkers; Carcinoma, Squamous Cell; Humans; Killer Cells, Natural; Lymphocyte Subsets; Male; Middle Aged; Mouth Neoplasms; Smoking; Taiwan
Publisher
Elsevier B.V.
Type
journal article