N-phenacylthiazolium bromide inhibits the advanced glycation end product (AGE)-age receptor axis to modulate experimental periodontitis in rats
Journal
Journal of Periodontology
Journal Volume
85
Journal Issue
7
Pages
e268-e276
Date Issued
2014
Author(s)
Abstract
Background: Advanced glycation end products (AGEs) are involved in the inflammatory process and are considered to be etiologic factors of diabetic periodontitis. The purpose of this study is to investigate the capability of Nphenacylthiazolium bromide (PTB), a glycated cross-link breaker, in the modulation of periodontitis in various disease phases. Methods: Mitogenesis and cytotoxicity of human periodontal ligament cells (hPDLCs) undergoing PTB treatment were evaluated in vitro. In vivo biomodulation was investigated by systemically administering PTB in the induction, progression, and recovery phases of ligature-induced periodontitis in rats, with the results evaluated by microcomputed tomography, histology, immunohistochemistry of the AGE and AGE receptor (RAGE), and gene expression of tumor necrosis factor-α (TNF-α), RAGE, periostin, fibronectin, and type I collagen. Results: Significantly promoted mitogenesis and reduced cytotoxicity of hPDLCs were noted with 0.05 to 0.1 mM PTB treatment at 24 hours. Systemic PTB administration significantly reduced periodontal bone loss, AGE deposition, and expressions of TNF-α and RAGE but elevated the periostin level in all three phases of periodontitis. Conclusion: PTB inhibits the induction and progression of periodontitis and facilitates its recovery via improving cellular viability and inhibiting the AGE-RAGE axis.
Subjects
Gene expression; Glycosylation end products, advanced; Immunohistochemistry; Periodontitis; Thiazoles; X-ray microtomography
SDGs
Other Subjects
advanced glycation end product; advanced glycosylation end-product receptor; cell adhesion molecule; collagen type 1; fibronectin; glucose blood level; glycosylated hemoglobin; immunoglobulin receptor; N-phenacylthiazolium bromide; Postn protein, rat; thiazole derivative; tumor necrosis factor alpha; alveolar bone loss; analysis; animal; antagonists and inhibitors; cell line; cell survival; culture technique; cytology; disease course; disease model; drug effects; glucose blood level; human; immunohistochemistry; male; periodontal ligament; periodontitis; rat; Sprague Dawley rat; Alveolar Bone Loss; Animals; Blood Glucose; Cell Adhesion Molecules; Cell Culture Techniques; Cell Line; Cell Survival; Collagen Type I; Disease Models, Animal; Disease Progression; Fibronectins; Glycosylation End Products, Advanced; Hemoglobin A, Glycosylated; Humans; Immunohistochemistry; Male; Periodontal Ligament; Periodontitis; Rats; Rats, Sprague-Dawley; Receptors, Immunologic; Thiazoles; Tumor Necrosis Factor-alpha
Publisher
American Academy of Periodontology
Type
journal article