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  3. School of Dentistry / 牙醫專業學院
  4. Clinical Dentistry / 臨床牙醫學研究所
  5. Arecoline inhibits endothelial cell growth and migration and the attachment to mononuclear cells
 
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Arecoline inhibits endothelial cell growth and migration and the attachment to mononuclear cells

Journal
Journal of Dental Sciences
Journal Volume
9
Journal Issue
3
Pages
258-264
Date Issued
2014
Author(s)
Tseng S.-K.
Chang M.-C.
Hsu M.-L.
Su C.-Y.
Chi L.-Y.
Lan W.-C.
JIIANG-HUEI JENG  
DOI
10.1016/j.jds.2012.12.015
URI
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84924021814&doi=10.1016%2fj.jds.2012.12.015&partnerID=40&md5=4f128f7f72e13d65eee173533ba07624
https://scholars.lib.ntu.edu.tw/handle/123456789/570654
Abstract
Background/purpose Betel quid (BQ) chewing is a popular habit in South-Asian and Southeast Asian countries, and Taiwan. BQ chewing can cause oral cancer and oral submucous fibrosis, and increases the risk of cardiovascular diseases. However, how BQ chewing affects endothelial cells and is involved in cardiovascular diseases and vascular changes is not fully understood. The effects of arecoline, a component of BQ, on the growth and migration of endothelial cells (EAhy 926), and their adherence by U937 cells were investigated. Materials and methods EAhy926 endothelial cells were cultured and exposed to various concentrations of arecoline for 24 hours. Morphological changes were observed using phase-contrast microscopy. Cytotoxic effects were analyzed using a 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. A wound closure assay was used to evaluate the cellular migration of EAhy926 cells. The attachment of 2′,7′-bis-(2-carboxyethyl)-5-(6)- carboxyfluorescein-labeled U937 cells to EAhy926 endothelial cells that were pretreated with various concentrations of arecoline was further studied. Results The addition of arecoline at concentrations >0.4 mM significantly decreased cellular viability. EAhy endothelial cells showed marked morphological changes, and cellular migration decreased after 24 hours and 48 hours of exposure to arecoline. The number of U937 cells attached to EAhy 926 cells increased when endothelial cells were pretreated by arecoline. Conclusion Arecoline impaired vascular endothelial cells by inhibiting their growth and migration and their adhesion to U937 mononuclear cells. These results reveal that arecoline may contribute to the pathogenesis of oral submucous fibrosis and cardiovascular diseases by affecting endothelial cell function in BQ chewers. ? 2012, Association for Dental Sciences of the Republic of China. Published by Elsevier Taiwan LLC. All rights reserved.
Subjects
arecoline; betel quid; cardiovascular diseases; endothelial cells; oral cancer; oral submucous fibrosis
SDGs

[SDGs]SDG3

Publisher
Association for Dental Sciences of the Republic of China
Type
journal article

臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of “NTU Repository” with “Academic Hub” to form NTU Scholars.

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開放取用是從使用者角度提升資訊取用性的社會運動,應用在學術研究上是透過將研究著作公開供使用者自由取閱,以促進學術傳播及因應期刊訂購費用逐年攀升。同時可加速研究發展、提升研究影響力,NTU Scholars即為本校的開放取用典藏(OA Archive)平台。(點選深入了解OA)

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