https://scholars.lib.ntu.edu.tw/handle/123456789/570655
標題: | Association of pocket epithelial cell proliferation in periodontitis with TLR9 expression and inflammatory response | 作者: | Chen, Yen-Chun CHEING-MEEI LIU JIIANG-HUEI JENG CHIA-CHI KU |
公開日期: | 2014 | 出版社: | Elsevier B.V. | 卷: | 113 | 期: | 8 | 起(迄)頁: | 549-556 | 來源出版物: | Journal of the Formosan Medical Association | 摘要: | Background/Purpose: Inflammatory response is triggered after recognition of microbial ligands by innate receptors such as Toll-like receptors (TLRs) and Nucleotide oligomerization domain (NOD)-like receptors (NLRs). In this study, we examined serial frozen sections of gingival biopsies from patients with gingivitis or periodontitis by immunohistochemical analysis for the topographic expression patterns of selected innate receptors and their association with cell proliferation in clinically healthy and diseased gingival tissues. Methods: A total of 19 gingival biopsies were collected from patients at the School of Dentistry, National Taiwan University Medical Center according to approved protocol and with informed consent. The specimens were assigned to either the gingivitis group or periodontitis group after clinical evaluation using gingival index. Frozen sections of gingival biopsies were stained with hematoxylin and eosin for histological evaluation. Serial sections of the same samples were stained with a panel of antibodies for immunohistochemical analysis. Expression of each protein marker was compared in the oral versus the sulcular epithelium of the same section. Results: Expression of cytokeratin 19 (CK19) was markedly increased in the basement membranes of the oral epithelium and in all layers of the pocket epithelium where it caused evident cell proliferation and migration of sulcular epithelial cells into the lamina propria of periodontitis tissue. TLR4 and the cytoplasmic NLRP3 were expressed in all sections examined regardless of disease state. However, expression of TLR9-, CK19- and collagenolytic matrix metalloproteinase-13 and activated NF-κB subunit p65 was more commonly found in periodontitis tissues than in gingivitis tissues. Conclusion: Activation of TLR9 signaling in the pocket epithelium was highly associated with periodontal inflammation and possibly with loss of tissue integrity. Further studies of mechanisms by which TLR9 signaling is activated in the periodontal epithelium may lead to new strategies for treating periodontitis. ? 2012. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84904395218&doi=10.1016%2fj.jfma.2012.07.043&partnerID=40&md5=4c8f27b69ca587e53d3a7467c79d283e https://scholars.lib.ntu.edu.tw/handle/123456789/570655 |
ISSN: | 0929-6646 | DOI: | 10.1016/j.jfma.2012.07.043 | SDG/關鍵字: | collagenase 3; cryopyrin; cytokeratin 19; eosin; hematoxylin; immunoglobulin enhancer binding protein; toll like receptor 4; toll like receptor 9; biological marker; TLR9 protein, human; toll like receptor 9; adult; aged; article; basement membrane; cell migration; cell proliferation; clinical article; controlled study; enzyme activation; epithelium cell; female; frozen section; gingival biopsy; gingivitis; human; human tissue; immunohistochemistry; lamina propria; male; mouth mucosa; periodontal pocket; periodontitis; protein expression; Taiwan; cell proliferation; epithelium; epithelium cell; genetics; gingiva; metabolism; middle aged; pathology; periodontitis; Adult; Aged; Biomarkers; Cell Proliferation; Epithelial Cells; Epithelium; Female; Gingiva; Gingivitis; Humans; Male; Middle Aged; Periodontitis; Taiwan; Toll-Like Receptor 9 |
顯示於: | 臨床牙醫學研究所 |
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