https://scholars.lib.ntu.edu.tw/handle/123456789/570695
標題: | Inhibitory effects of chitooligosaccharides on tumor growth and metastasis | 作者: | Shen K.-T. Chen M.-H. Chan H.-Y. JIIANG-HUEI JENG Wang Y.-J. |
關鍵字: | Cell cycle; Chitooligosaccharides; SCID mice; Tumor growth; Tumor metastasis | 公開日期: | 2009 | 卷: | 47 | 期: | 8 | 起(迄)頁: | 1864-1871 | 來源出版物: | Food and Chemical Toxicology | 摘要: | Chitooligosaccharides (COS) are hydrolyzed products of chitosan and have been proven to exhibit various biological functions. The objectives of this study were to evaluate the anti-tumor growth, anti-metastatic potency and related pathways of COS extracted from fungi. In in vitro studies, we found that COS significantly inhibited human hepatocellular carcinoma (HepG2) cell proliferation, reduced the percentage of S-phase and decreased DNA synthesis rate in COS-treated HepG2 cells. Expressions of cell cycle-related genes were analyzed and the results indicated that p21 was up-regulated, while PCNA, cyclin A and cdk-2 were down-regulated. Moreover, we also found that the activity of metastatic related protein (MMP-9) could be inhibited by COS in Lewis lung carcinoma (LLC) cells. In in vivo studies, we found that COS inhibited the tumor growth of HepG2 xenografts in severe combined immune deficient (SCID) mice. In a LLC-bearing mouse tumor growth and lung metastasis model, COS inhibited tumor growth and the number of lung colonies in LLC-bearing mice as well as the lung metastasis, and it prolonged the survival time of the LLC-mice. These results suggest a potential anti-tumor growth and anti-metastatic potency of COS in cancer chemoprevention. ? 2009 Elsevier Ltd. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-68649097772&doi=10.1016%2fj.fct.2009.04.044&partnerID=40&md5=d069db708bd00798e2a5a8be2f4245d2 https://scholars.lib.ntu.edu.tw/handle/123456789/570695 |
ISSN: | 0278-6915 | DOI: | 10.1016/j.fct.2009.04.044 | SDG/關鍵字: | chitooligosaccharide derivative; chitosan; cyclin A; cyclin dependent kinase 2; cycline; gelatinase B; protein p21; unclassified drug; animal experiment; animal model; antineoplastic activity; article; cancer inhibition; cell cycle S phase; cell proliferation; cell strain HepG2; controlled study; DNA synthesis inhibition; down regulation; enzyme activity; gene expression; human; human cell; in vitro study; in vivo study; Lewis carcinoma; lung metastasis; male; metastasis inhibition; mouse; nonhuman; SCID mouse; survival time; tumor model; tumor xenograft; upregulation; Animals; Antineoplastic Agents; Blotting, Western; Cell Cycle; Cell Line, Tumor; Chitosan; Coloring Agents; Gelatin; Humans; Matrix Metalloproteinases; Mice; Mice, Inbred C57BL; Mice, SCID; Molecular Weight; Neoplasm Metastasis; Neoplasms; Oligosaccharides; Tetrazolium Salts; Thiazoles; Fungi; Mus |
顯示於: | 臨床牙醫學研究所 |
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