https://scholars.lib.ntu.edu.tw/handle/123456789/571314
標題: | Programmed −1 ribosomal frameshifting from the perspective of the conformational dynamics of mRNA and ribosomes | 作者: | Chang, Kai Chun JIN-DER WEN |
關鍵字: | Cryo-EM | MD simulation | Optical tweezers | Ribosomal frameshifting | Single-molecule | smFRET | 公開日期: | 1-一月-2021 | 出版社: | Elsevier B.V. | 卷: | 19 | 起(迄)頁: | 3580-3588 | 來源出版物: | Computational and Structural Biotechnology Journal | 摘要: | Programmed −1 ribosomal frameshifting (−1 PRF) is a translation mechanism that regulates the relative expression level of two proteins encoded on the same messenger RNA (mRNA). This regulation is commonly used by viruses such as coronaviruses and retroviruses but rarely by host human cells, and for this reason, it has long been considered as a therapeutic target for antiviral drug development. Understanding the molecular mechanism of −1 PRF is one step toward this goal. Minus-one PRF occurs with a certain efficiency when translating ribosomes encounter the specialized mRNA signal consisting of the frameshifting site and a downstream stimulatory structure, which impedes translocation of the ribosome. The impeded ribosome can still undergo profound conformational changes to proceed with translocation; however, some of these changes may be unique and essential to frameshifting. In addition, most stimulatory structures exhibit conformational dynamics and sufficient mechanical strength, which, when under the action of ribosomes, may in turn further promote −1 PRF efficiency. In this review, we discuss how the dynamic features of ribosomes and mRNA stimulatory structures may influence the occurrence of −1 PRF and propose a hypothetical frameshifting model that recapitulates the role of conformational dynamics. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/571314 | ISSN: | 20010370 | DOI: | 10.1016/j.csbj.2021.06.015 |
顯示於: | 分子與細胞生物學研究所 |
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