Regulation of metE+ mRNA expression by FnrS small RNA in Salmonella enterica serovar Typhimurium
Journal
Microbiological Research
Journal Volume
229
Date Issued
2019
Author(s)
Chien C.-W
Chan Y.-F
Shih P.-S
Kuan J.-E
Wu K.-F
Wu C
Wu W.-F.
Shih, Po Shu
WHEI-FEN WU
Chien, Chia Wei
Wu, Cindy
Kuan, Jung En
Chan, Yu Feng
Abstract
Methionine is critical for variety of metabolic processes in biological organisms, acting as a precursor or intermediate for many final products. The last step for the synthesis of methionine is the methylation of homocysteine, which is catalyzed by MetE. Here, we use Salmonella enterica serovar Typhimurium LT2 to study the regulation of the metE+ gene by an anaerobically induced small non-coding RNA-FnrS, the expression of which is strictly dependent on the anaerobic regulator-FNR. The MetE-HA protein was expressed at an increased level in the fnrS- and hfq- deficient strains under anaerobic conditions. The Hfq protein is predicted to stabilize the binding between small RNA(s) and their target mRNA(s). A transcriptional (op) and translational (pr) metE
Subjects
Alkylation; Amino acids; Polymerase chain reaction; Proteins; Restoration; RNA; Transcription; FnrS; metE^+; Methionine; Non-coding RNAs; Salmonella enterica serovar Typhimurium; Salmonella; amino acid; bacterium; chemical binding; gene; gene expression; metabolism; methylation; protein; RNA; Salmonella enterica subsp. enterica serovar Typhimurium; bacterial protein; bacterial RNA; messenger RNA; methyltransferase; small untranslated RNA; base pairing; chemistry; conformation; enzymology; gene expression regulation; genetics; metabolism; nucleotide sequence; Salmonella enterica serovar Typhimurium; Bacterial Proteins; Base Pairing; Base Sequence; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Methyltransferases; Nucleic Acid Conformation; RNA, Bacterial; RNA, Messenger; RNA, Small Untranslated; Salmonella typhimurium
SDGs
Type
journal article